2010-01-012024-05-15https://scholars.lib.ntu.edu.tw/handle/123456789/662719摘要：生醫分子影像核心實驗室的主要目標在於提供活體研究方式以探索關鍵基因在特定生物性程序中的活體功能。基本的想法是藉由通道分析決定在生物性程序中真正的關鍵基因。一旦這些關鍵基因被確認，我們將設計目標特定的分子探針以觀察這些關鍵基因的活體功能。藉由將探針接在特定的基因產物，我們將以快速高解析度的核磁共振影像、超音波影像以及三維光學影像，觀察這些關鍵基因的活體活動。此外，生醫分子影像之平台建立可以提供台大醫院及總區有關動物體疾病模式的早期偵測、診斷及治療之相關領域的研究及服務，並可同時提供分子與細胞內次細胞微結構與功能的動態影像。此平台更能達到長期動態偵測特定基因在活體上的表達，以了解不同時期、不同環境下不同的基因表達情形。提供活體下探討基因間相互作用的一項可行工具，以滿足後基因時代之分子生物、細胞生物與發展生物醫學之整合研究需要。本核心實驗室亦計畫結合奈米醫藥、抗體工程之相關技術，進行多功能性之對比顯影劑以利於從事跨模態研究，亦對於幹細胞之標定追蹤提供相關之研究平台。 本核心實驗室的研究方向及目標，如下所示： &#8226; 建立細胞微陣列之研究平台於功能性基因體之相關研究。 &#8226; 提供高效率及高品質之基因遞送之相關製劑。 &#8226; 建立活體內之基因遞送效率及基因表現之監測平台。 &#8226; 運用基因操作和抗體工程製備技術來應用於細胞膜表面生物標記之抗體或蛋白質研究。 &#8226; 設計並置備可運用於不同分子影像擷取平台之具有生物相容性及標定功能之奈米藥物或分子探針。 &#8226; 提供應用於基因表達並藉由特定標定之活體影像觀測的快速且高解析磁振造影技術平台。 &#8226; 建立以微脂體作為顯影製劑之顯微超音波生醫分子影像技術平台。 &#8226; 建立活體內之幹細胞分化、追蹤之觀測平台。 &#8226; 提供高解析之三維光學成像技術平台於活體胚胎基因表達之相關領域研究。 &#8226; 提供活體內癌症細胞長期連續觀測與追蹤之技術平台。 &#8226; 高穿透度細胞內分子生命期動態觀測。 &#8226; 建構校園內之奈米醫藥相關研究教學網絡。<br> Abstract: The biomedical molecular imaging core facility aims to offer an in vivo approach to investigating the functions of critical genes in a biological process. The idea is to determine the true critical genes in a biological process through pathway analysis. Once these critical genes have been identified, target-specific probes will be designed for the observation of the in vivo functions of these critical genes. By tailoring the probes to the specific gene products, the activities of these critical genes may be in vivo observed through fast high-resolution MRI, ultrasound imaging and 3D optical imaging. Furthermore, the platform of biomedical molecular imaging development could provide the clinical diagnosis, therapy researches and service in animal disease model for NTU, and can also provide dynamic molecular imaging with a sub-micron spatial resolution and high cell viability and can provide high correlation with other imaging modalities. This platform could monitor the specific gene expression in vivo to distinguish the different behaviours of gene in different environment or different stage. It is a highly potential tool to research the different gene interaction in vivo. On the other hand, the core facility also combined with nanotechnology and nanomedicine research, we will also developing multimodality contrast agents for intravital correlation imaging. Furthermore, this core lab also provide the research and the platform of imaging modalities for stem cell targrting and tracking. The specific aims and research goals of biomedical molecular imaging core facility are: &#8226; To establish cell arrays platform for functional genomics analysis. &#8226; Providing high quality reagents for gene delivery. &#8226; To monitor the efficiency of gene delivery and gene expression in vivo. &#8226; To generate antibodies against target proteins on cell membrane. &#8226; To design and functionalize the biocompatible nanoparticles or molecular probes for imaging modalities. &#8226; Providing fast high-resolution MR imaging facility to observe in vivo gene expressions labelled by the target-specific probes. &#8226; Developing ultrasonic micro-imaging technologies for detecting liposome-based molecular probes. &#8226; To monitor stem cells differentiation and fate in vivo. &#8226; Providing high-resolution 3D optical imaging facility to observe in vivo gene expressions. &#8226; Dynamically and long term intravital cancer cell diagnosis and precancer cells imaging. &#8226; Intravital molecular fluorescence lifetime imaging. &#8226; To construct nanomedicine website on campus.