2018-08-012024-05-17https://scholars.lib.ntu.edu.tw/handle/123456789/672155摘要:本計畫主要目的為探索印度-西太平洋區之巽他陸棚屏障 (Sunda Shelf barrier, SSB)對海洋魚類演化及地理分布的作用。SSB 是熱帶海洋主要的生物地理屏障之一,它使得兩大洋(印度洋和太平洋)之生物相有所分離。然而,不同的相關研究所推論的生物地理分界位置或作用有不一致的結果,因此仍有需要進一步從不同的動物類群涉及生物地理學的研究調查。在此於本計畫,我們在方法之取樣策略上採取兩種不同層級的方式去做分析及進行研究。首先我們先選定分布於印度-西太平洋區之中心海域但分布橫跨SSB (至少部分魚種)的鱸形目石首魚科(Sciaenidae)之白姑魚類(Pennahia spp.)作為本課題之案例, 並以整合式的分析策略,結合由古典的 (Sanger) 及最先進的次世代測序方法得到的研究樣本DNA序列變異進行分析,以研究所選定之白姑魚屬內各不同魚種自身的族群遺傳結構,親緣地理結構和種間之親緣關係。此外,為探討居住在此海域之海洋魚類對其生物地理屏障(SSB)之回應是否因生物本身的不同生態習性有關聯性,我們採取完整的取樣策略,包括在生態上及演化上不同類群的海洋魚類,並以DNA-barcoding 方法進行分析,以進一步了解海洋魚類在此海域之生物多樣性呈現,是否為該區複雜的地質歷史、海洋環境或生態因子所造成。此外,並預期發 現新種或隱蔽種之可能性。<br> Abstract: The Sunda Shelf barrier (SSB) is one of the major tropical marine biogeographic barriers and separates biota from two major ocean basins (Indian and Pacific). However, the effect of SSB was often questioned, as the localities of the biogeographic breaks were found inconsistent or no breaks at all. Advanced comparative surveys of species from various zoological groups are still needed to evaluate the role of the SSB on the “genealogical” structure of marine species inhabiting the Indo-West Pacific, particularly in Indomalayan realm. In this project, we used molecular approaches in systematic biology to 1.test the significance of the SSB to evolution of the marine fishes; 2.examine the influence of different ecological or habitat preference on the contemporary patterns of genetic diversity and divergence; 3.investigate the hidden diversity of marine fishes in this region in order to have better insight and accurate diversity information.生物多樣性生物地理印度-西太平洋演化生物條碼biodiversitybiogeographyIndo-PacificEvolutionDNA-Barcoding人力結構改善/海洋所/Testing the significance of the Sunda Shelf barrier to the evolution of marine fishes in Indomalayan realm