2017-07-012024-05-17https://scholars.lib.ntu.edu.tw/handle/123456789/681945摘要：番茄在農業與科研上皆極為重要，但因極易遭受多種病菌侵害，包括由細菌Ralstonia solanacearum (Rs) 引起之致死青枯病 (bacterial wilt，簡稱BW)，造成番茄等許多重要經濟作物的嚴重損失。此外，植物病害反應與非生物逆境亦有交互作用。有些番茄品系具有各式防禦機制而得以存活，而深入瞭解並善用作物天然抗病資源便至關重要，尤其是對於研擬對環境友善之防治措施更是如此。我們目前的研究結果發現，在番茄與菸草表現一個青枯病菌之效應蛋白(effector)可以提升植物對抗三種特性迥異之重要病原菌: 青枯病菌 (Rs)、細菌性軟腐病菌 (Pectobacterium carotovorum subsp. carotovorum) 及真菌灰黴病菌 (Botrytis cinerea)。這些發現顯示病菌效應蛋白所誘導之植物保護措施具重要性與新穎性，然而其具體調控機制尚待闡明。基於這些基礎以及我們已經建立之高效研究專長與資源，本研究將: (I) 分析此效應蛋白所誘導之植物保護效應對於其他重要病害與非生物性逆境之有效範圍； (II) 闡述其防禦訊息傳調路徑； (III) 找尋與其具直接交互作用與被調控之目標基因/蛋白質以瞭解其具體調控機制。藉由此整合型計畫，本研究預期將獲得植物防禦機制之重要資訊，並匯集可能有助於作物農業生技發展之資源。<br> Abstract: Tomato is a globally important vegetable and has been reputed as a useful study system for crop researches. However, tomato production losses due to various diseases, including the deadly bacterial wilt caused by the devastating pathogen Ralstonia solanacearum (Rs), have been very huge. In addition, plant disease responses often share certain common features and have cross-talks with abiotic stresses. Some tomato cultivars have evolved to equip with versatile defense mechanisms and sustain their life. Exploring and using natural crop defense resources to develop efficient disease and stress control strategies will be very valuable, particularly for establishing environmentally friendly crop-protection systems. Our current results showed that the expression of an Rs effector protein in tomato and tobacco plants promoted defense against three distinct important pathogens: Rs, Pectobacterium carotovorum subsp. carotovorum and Botrytis cinerea. These noteworthy findings highlight the importance and novelty of this effector-induced plant protection strategy; however, the involved regulatory mechanisms remained to be elucidated. Building on these bases and with the highly efficient functional genetic approaches and bioassay systems well-established in our group, this study aims: (I) To determine the spectrum of the effector-induced plant protection against additional important crop pathogens and abiotic stresses; (II) To decipher the involved defense signaling pathways; (III) To decipher the underlying regulatory mechanisms by identifying the interactors and regulated genes/targets of the effector. Through the integration of this Programmatic Project, the proposed studies are projected to gather important information on plant defense mechanisms and collect resources valuable for crop biotechnology.植物抗病植物逆境細菌效應蛋白plant disease defenseplant stressbacterial effector