王至弘2006-07-262018-07-132006-07-262018-07-132004http://ntur.lib.ntu.edu.tw//handle/246246/26359Poly (lactic acid) is a biodegradable biomedical material that has been used for bone and cartilage repair. Biomaterials coated with various ECM have been shown to promote cell adhesion, proliferation, and differentiation. The purpose of this study is to develop scaffolds that are suitable with ligament cells attachment and to use ligament cells derived from bone marrow stem cells to induce tissue regeneration. The first thing of this project is to examine the cellular behaviors of ligament on various ECM coated on three kinds of PLLA membranes. Before the proceeding of the co-culture system of mesenchymal stem cell (MSC) and ligament cells, we develop some method to differentiate BMSC from ligament cells. First we used the flow cytometry to determine a lot of surface antigens of mesenchymal stem cell like CD105, CD29, CD44, CD71, and CD34. The differentiated fibroblasts are examined by scanning electron microscopy, immunostaining of α- smooth muscle antibody. Furthermore, we develop a novel electrophoretic analysis for characterization. Since there are no definite markers for fibroblasts in the literature, our unique study will provide important information for further characterization of ligament or tendon fibroblasts.application/pdf845734 bytesapplication/pdfzh-TW國立臺灣大學醫學院骨科journal articlehttp://ntur.lib.ntu.edu.tw/bitstream/246246/26359/1/922320B002106.pdf