Sie, Zong HanZong HanSieWu, Lu WeiLu WeiWuChang, Yen YuYen YuChangToh, Yi ChinYi ChinTohYA-YU CHIANG2023-09-222023-09-222020-01-019781733419017https://scholars.lib.ntu.edu.tw/handle/123456789/635647The complexity of syringe-based or vacuum-driven micropumping requires intricate experimental settings to perform the experiments, consequently diminishing its applicability. Here, we developed a pump-free microfluidic chips to simplify the structure of biochips and improve the flow stability during in situ cell-culture using osmotic pressure. The osmotic pressure drives culture medium smoothly in the microchannel, creating a constant shear stress that endothelial cells experience similarly in human micro-vessels. The aligned arrangements of endothelial cells under tunable shear stress would be presented.Cell arrangement | Osmotic pressure | Shear stressconference paper2-s2.0-85098266333https://api.elsevier.com/content/abstract/scopus_id/85098266333