Endo, MasakiMasakiEndoMikami, MasafumiMasafumiMikamiEndo, AkiraAkiraEndoKaya, HidetakaHidetakaKayaTAKESHI ITOHNishimasu, HiroshiHiroshiNishimasuNureki, OsamuOsamuNurekiToki, SeiichiSeiichiToki2024-01-292024-01-292019-01-012055-0278https://www.scopus.com/inward/record.uri?eid=2-s2.0-85058157282&doi=10.1038%2fs41477-018-0321-8&partnerID=40&md5=c7a8b99d252449123a682dd5b526828ahttps://scholars.lib.ntu.edu.tw/handle/123456789/639064Streptococcus pyogenes Cas9 (SpCas9) is widely used for genome editing and requires NGG as a protospacer adjacent motif (PAM). Here, we show that the engineered SpCas9 (SpCas9-NGv1) can efficiently mutagenize endogenous target sites with NG PAMs in the rice and Arabidopsis genomes. Furthermore, we demonstrate that the SpCas9-NGv1 nickase fused to cytidine deaminase mediates C-to-T substitutions near the 5′ end of the target sequence.enjournal article10.1038/s41477-018-0321-8305319392-s2.0-85058157282https://api.elsevier.com/content/abstract/scopus_id/85058157282