胡文聰臺灣大學：應用力學研究所羅英傑Lo, Ying JieYing JieLo2007-11-292018-06-282007-11-292018-06-282004http://ntur.lib.ntu.edu.tw//handle/246246/62349本論文利用介電物質材料特性在交流電場下所形成介電泳力，作為血球血漿分離及血球操控運用。介電泳基礎在於粒子與溶劑之間，介電能力及導電能力的差異性誘導出一個決定於頻率高低、電場空間分佈及相位空間分佈且具方向性之力量。 透過微機電曝光、顯影等製程達到元件微小化及提高介電泳力量達到影響粒子運動，並且透過基本的數值分析計算求出電場分佈及血球軌跡以作為元件設計的依據。利用PDMS高生物親和力材料作為流道製程之材料以便未來的整合。 設計出之血球血漿分離方式有階梯型、傾斜型、梯度分佈型電極與三維流道輔助等，而在粒子操控方面旅波電泳於血球之利用、血球集中元件、血球分配元件均試驗成功。This thesis takes advantage of dielectric property of material, where a dielectrophoretic (DEP) force is induced in a sinusoidally time-variant electric field to achieve cell-plasma separation and cell manipulation. Dielectrophoretic theory is based on the distinct dielectric and conductive properties of cell and medium. This distinction will physically induce a directional force depending on frequency, spatial electric strength, and spatial electric phase distribution. Through fabrication of MEMS, devices miniaturized are to increase the influence of DEP force on separation and manipulation of cells. Additionally, with the aid of numerical simulation of electric field and cell trajectory, more effective devices are designed. The use of bio-compatible material polydimethysilloxane (PDMS) proved ease of fabrication and integration [1]. Types of cell-plasma separator tested various electrode design include stair, inclined, and gradient confuguration, and 3D channel assisting design. For cell manipulation, traveling wave, cell concentrator, and cell portioning devices are all tested and their performance quantified. Results show successful separation of red blood cell (RBC) and plasma vis DEP. for a wide range of electrode geometry configurations. Traveling wave DEP, however, was more difficult to implement. Manipulation of RBC proved viable using the non-uniform E-field at the tip of multi-electrode design.Chapter 1 Introduction 1.1 Research background and motivation 7 1.2 Separation mechanisms of cells and particles 8 1.3 Paper review 9 Chapter 2 Theory of Electromagnetic and Fluid Mechanics of Cells and Particles 2.1 Properties of cells, particles, and medium 12 2.1.1 Dielectric Constant and conductivity 12 2.2 Electromagnetic mechanics 17 2.2.1 Electrodynamics and electrostatics 17 2.2.2 characteristic time of electric field 18 2.2.3 characteristic length of electric field 18 2.3 Fluid mechanics of cells and particles 21 2.4 The governing equation of cells 22 Chapter 3 Simulation 3.1 Simulation tools 23 3.1.1 Matlab 23 3.1.2 Ansys 23 3.2 Governing equations 23 3.3 Numerical methods 24 3.3.1 Initial value problem 24 3.3.1.1 Euler method 24 3.3.1.2 Runge-Kutta method 25 3.3.2 Boundary value problem 26 3.3.2.1 Finite difference methods 36 Chapter 4 Experiments and Fabrication 4.1 Materials for the device 28 4.1.1 Cr or Al for electrode material 28 4.1.2 PDMS as micro-channel material 29 4.2. Device Fabrication 31 4.2.1 Electrode Fabrication 31 4.2.2 Channel Fabrication 32 4.2.3 Sub-device bonding 34 4.3 Environment setup 35 Chapter 5 Results and Discussion 5.1 Experiment and simulation of fundamental elements 36 5.1.1 cDEP on parallel electrodes 36 5.1.2 twDEP on parallel electrodes 39 5.2 Applications basing on cDEP and twDEP 46 5.2.1 Cell and plasma separation device 46 5.2.1.1 Step electrode design 46 5.2.1.2 Red blood cell and plasma separation on an inclined electrode design 48 5.2.1.3 A spatial gradient distribution for blood and plasma separation 51 5.2.1.4 Cell and plasmas separate through a 3D-channel 51 5.2.2 Cell concentration and manipulation device 54 5.2.3 Cell portion device 55 5.3 Other experiment 56 Chapter 6 Conclusion and Future work 58 Reference 591523684 bytesapplication/pdfen-US介電泳血球blood celldielectrophoresisthesishttp://ntur.lib.ntu.edu.tw/bitstream/246246/62349/1/ntu-93-R91543048-1.pdf