闕玲玲2006-07-262018-07-092006-07-262018-07-092002http://ntur.lib.ntu.edu.tw//handle/246246/28707雞貧血病毒VP3 基因轉譯之蛋白質， 又稱為細胞凋零素，已被證明可以誘發多 種人類惡性或轉型的細胞株發生細胞凋 零，但對正常細胞卻不會引起傷害，因此 使其成為目前研究抗腫瘤治療之新方向。 為追蹤VP3 基因之表現並探討其做為動物 腫瘤基因治療之潛力，本研究以選殖自台 灣宜蘭分離株雞貧血病毒之VP3 基因，建 構真核表現載體，於犬乳房腫瘤之細胞中 進行表現此蛋白之表現。追蹤表現細胞發 現此蛋白先出現於細胞質，隨後逐漸聚集 於核內，於表現48 小時開始即可見部分細 胞出現核濃縮及破裂等細胞凋零之典型特 徵，而隨著表現時間之增長，於72 小時左 右細胞發生死亡。由以上結果顯示VP3 蛋 白，確實具有開發為一犬隻乳房腫瘤基因治療藥物之潛力。VP3 protein of chicken anemia virus has been reported to induce apoptosis in transformed and malignant cells but not in normal diploid or primary cells. This tumor-specific selective killing activity of VP3 makes it an attractive candidate for cancer gene therapy. To explore the possibility of using VP3 protein as an anti-tumor agent in veterinary medicine, a cloned VP3 gene from a Taiwanese isolate was constructed into eukaryotic expression vectors and its effect on the cells originated from a canine mammary gland tumor was studied. The protein was first found distributed in cytoplasm and later aggregated in the nuclei. Cells expressing VP3 protein underwent morphological changes and died at a later stage after transfection. The cells which expressed VP3 protein showed apoptosis specific changes, e.g. shrinkage of cells, segmentation of the nucleus, condensation and degradation of DNA at 72 hours posttransfection. Taken together, the data demonstrate that VP3 may constitute a promising agent for the treatment of canine mammary gland tumor.application/pdf2222878 bytesapplication/pdfzh-TW國立臺灣大學獸醫學系暨研究所雞貧血病毒VP3 蛋白細胞凋零基因治療chicken anemia virusVP3 proteinapoptosisgene therapy[SDGs]SDG3reporthttp://ntur.lib.ntu.edu.tw/bitstream/246246/28707/1/902313B002310.pdf