國?臺灣大學生態學與演化生物學研究所; 國?臺灣大學生態學與演化生物學研究所; 國?東華大學自然資源管?研究所Institute of Ecology and Evolutionary Biology, National Taiwan University; Institute of Ecology and Evolutionary Biology, National Taiwan University; Institute of Natural Resources, National Dong Hwa University儲瑞華?曜松吳海音Chu, J.H.J.H.ChuLin, Y.S.Y.S.LinWu, H.Y.H.Y.Wu2017-09-122018-07-062017-09-122018-07-062006-12http://ntur.lib.ntu.edu.tw//handle/246246/283493本文為檢驗非侵?性採樣法在臺灣獼猴族群遺傳研究應用性的前導實驗。本研究採集獼猴排遺樣本作為非侵?性之DNA 樣本?源，並?用微隨體及?線體DNA ?種分子標幟檢驗其PCR 成功?。在與其他哺乳動物非侵?性遺傳研究的比較後發現，臺灣獼猴的微隨體PCR 成功?較低（42.4%, N = 181），而?線體DNA 的PCR 成功?則尚可接受（66.5%，N = 334）。微隨體對偶基因的低PCR 成功?及低可重複性（即高對偶基因遺失?及高假性對偶基因出現?），使得可靠的微隨體資?庫難以建?。然而，日新月?的實驗技術也許能克服以上的實驗困境。This paper presents a pilot study conducted to test the applicability of non-invasive sampling approach in population genetic studies of Taiwanese macaques (Macaca cyclopis). Monkey feces were collected in the field and used as non-invasive DNA sources. PCR success rates of both microsatellite and mitochondrial DNA markers were examined. When compared with other studies by non-invasive genetic sampling of different mammal species, success rate of microsatellite PCR amplification is low (42.4%, N = 181) while that of mtDNA PCR amplification is acceptable (66.5%, N = 334). The low PCR success rate and poor PCR repeatability of microsatellite alleles due to allelic dropout and false alleles make it difficult to obtain a reliable microsatellite data set. However, the difficulties may be overcome by new techniques.721808 bytesapplication/pdfMacaca cyclopisNon-invasive samplingMicrosatellitemtDNA d-loop臺灣獼猴非侵?性採樣法微隨體?線體DNA d-loopjournal articlehttp://ntur.lib.ntu.edu.tw/bitstream/246246/283493/1/5104_200612_2.pdf