Organic anion-transporting polypeptide 1B3 as a dual reporter gene for fluorescence and magnetic resonance imaging

Wu M.-R.HON-MAN LIULu C.-W.Shen W.-H.Lin I.-J.Liao L.-W.YI-YOU HUANGMING-JIUM SHIEHHsiao J.-K.2020-08-212020-08-2120180892-6638https://scholars.lib.ntu.edu.tw/handle/123456789/513347Reporter proteins have broad applications in visualizing molecular events at the cellular, tissue and whole-body levels. Transmembrane transporters recognizing specificmolecular domains are of particular interest because they enable the migration of signal-source molecules from the extracellular space to the cytoplasm for subsequent application in multimodality imaging. Organic anion-transporting polypeptides (OATPs) have demonstrated their MRI reporter efficacy. We further expanded their use as a dual-modality reporter in MRI and noninvasive in vivo imaging system (IVIS). We overexpressed OATP1B3 in the HT-1080 sarcoma cell line. Both Gd-EOB-DTPA, anMRI contrast agent, and indocyanine green (ICG), a near-infrared fluorescent dye that provides better deep-tissue detection because of its longwavelength, couldbe deliveredto the intracellular space and imaged in a tumor-bearing nudemousemodel. Our in vivo dual-imaging reporter systemachieved high sensitivity inMRI and observation periods lasting as long as 96 h in IVIS. Because of the superior temporal and spatial resolutions and the clinical availability of both ICG and Gd-EOB-DTPA, this dual-imaging OATP1B3 systemwill find biomedical use in tumor biology, stemcell trafficking, and tissue engineering. ? The Author(s).[SDGs]SDG3gadoxetic acid; indocyanine green; solute carrier organic anion transporter 1B3; SLCO1B1 protein, human; solute carrier organic anion transporter 1B1; animal cell; Article; cell migration; controlled study; female; fluorescence; HT-1080 cell line; in vivo study; intracellular space; mouse; nonhuman; nuclear magnetic resonance imaging; priority journal; protein expression; reporter gene; stem cell; tissue engineering; animal; Bagg albino mouse; cancer transplantation; diagnostic imaging; fluorescence imaging; genetics; human; metabolism; nude mouse; sarcoma; tumor cell line; xenograft; Animals; Cell Line, Tumor; Genes, Reporter; Heterografts; Humans; Magnetic Resonance Imaging; Mice; Mice, Inbred BALB C; Mice, Nude; Neoplasm Transplantation; Optical Imaging; Sarcoma; Solute Carrier Organic Anion Transporter Family Member 1b1Organic anion-transporting polypeptide 1B3 as a dual reporter gene for fluorescence and magnetic resonance imagingjournal article10.1096/fj.201700767R291467312-s2.0-85043571901