WANG-HUEI SHENGCHIEN-CHING HUNGHUAI-HSUAN HUANGLiang S.-Y.Cheng Y.-J.Ji D.-D.SHAN-CHWEN CHANG2020-12-302020-12-3020090002-9637https://www.scopus.com/inward/record.uri?eid=2-s2.0-68549106000&doi=10.4269%2fajtmh.2009.81.277&partnerID=40&md5=f7a28ffd92609fa67344033796632064https://scholars.lib.ntu.edu.tw/handle/123456789/535749We report the first case of granulomatous amebic encephalitis caused by Acanthamoeba in a previously healthy farmer in Taiwan who fell into a ditch. The DNA amplicons of amebas identified by polymerase chain reaction in the cerebrospinal fluid specimen and the ditch water specimens were identical and shared strong homology with A. castellanii. He survived after treatment with amphotericin B, rifampin, and corticosteroids. Copyright ? 2009 by The American Society of Tropical Medicine and Hygiene.[SDGs]SDG3amphotericin B; corticosteroid; cotrimoxazole; dexamethasone; DNA; flucytosine; furosemide; ketoconazole; mannitol; pentamidine; polymyxin; rifampicin; sulfadiazine; water; protozoal DNA; Acanthamoeba castellanii; adult; amplicon; article; case report; cerebrospinal fluid analysis; granulomatous amebic encephalitis; human; intracranial hypertension; male; nucleotide sequence; polymerase chain reaction; sequence homology; Taiwan; water pollution; amebiasis; animal; cerebrospinal fluid; encephalitis; genetics; granuloma; isolation and purification; middle aged; parasitology; pathology; phylogeny; Taiwan; Acanthamoeba; Acanthamoeba castellanii; Acanthamoeba castellanii; Amebiasis; Animals; DNA, Protozoan; Encephalitis; Granuloma; Humans; Male; Middle Aged; Phylogeny; Taiwanjournal article10.4269/ajtmh.2009.81.27719635883