2007-01-012024-05-17https://scholars.lib.ntu.edu.tw/handle/123456789/682508摘要：研發出茄科作物重要病毒診斷鑑定晶片，並將研究成果轉移給國內防疫與檢疫單位使用。本計畫今年將執行下列重要工作：自行設計並製備能感染茄科作物的tombusvirus之廣效性引子對和單股寡核酸探針，並將探針點漬在尼龍膜上，先以人工接種的植物病株為材料，測試所製備的核酸探針之專一性；再以田間採集的茄科作物為樣品，實際測試診斷鑑定晶片的效果。<br> Abstract: In order to develop an identification biochip for important solanaceous plant viruses and provide the chip to the authorities of plant inspection and quarantine, the following works will be executed this year. After collecting and analyzing the sequences of tombusviruses, the genus-specific degenerate primers and oligonucleotide probes of the viruses will be designed and prepared. By meaning of DIG-membrane system, the specificity of the probes will be tested on tombusvirus-inoculated plants. The effect of this identification biochip will be evaluated by crop samples collected from the field.生物晶片番茄馬鈴薯Y病毒屬菸草嵌紋病毒屬胡瓜嵌紋病毒屬番茄叢矮病毒屬biochiptomatopotyvirustobamoviruscucumovirustombusvirus