Liao C.H.Chou C.H.Lai C.C.YU-TSUNG HUANGTan C.K.Hsu H.L.PO-REN HSUEH2021-05-182021-05-1820090163-4453https://scholars.lib.ntu.edu.tw/handle/123456789/561177Objectives: To evaluate diagnostic performance of an enzyme-linked immunospot assay for interferon-γ (T SPOT-TB) in patients with suspected extrapulmonary tuberculosis (TB). Methods: From January 2007 to December 2008, patients with suspected extrapulmonary TB were prospectively enrolled from 2 tertiary care hospitals. Results: A total of 138 patients with suspected extrapulmonary TB were enrolled; 50 patients had positive culture for Mycobacterium tuberculosis and 39 patients had probable TB. The sites of infection were lymph node (n = 20), pleura (n = 19), bone/joint (n = 15), urinary tract (n = 7), peritoneum (n = 7), meninges (n = 6), disseminated (n = 5), intestine (n = 3), pericardium (n = 2), skin (n = 2), throat (n = 1), neck (n = 1), and genitalia (n = 1). The overall sensitivity and specificity were 79.8% (71/89) and 81.6% (40/49). The sensitivity ranged from 100% for tuberculous meningitis, tuberculous pericarditis, and intestinal TB, 95% for lymphadenitis, to 42.9% for tuberculous peritonitis. The sensitivity of the T?SPOT-TB assay was 70.6% in immunocompromised patients and 85.5% in immunocompetent patients (p = 0.09). Conclusions: The T SPOT-TB assay can be a useful tool for diagnosing extra-pulmonary TB in immunocompetent and immunocompromised patients, particularly for tuberculous meningitis, pericarditis, lymphadenitis, and intestinal TB. ? 2009 The British Infection Society.[SDGs]SDG3gamma interferon; adolescent; adult; aged; article; bacterium culture; controlled study; diagnostic value; enzyme linked immunospot assay; extrapulmonary tuberculosis; female; human; immunocompromised patient; intestine tuberculosis; major clinical study; male; Mycobacterium tuberculosis; predictive validity; sensitivity and specificity; tuberculous lymphadenitis; tuberculous meningitis; tuberculous pericarditis; tuberculous peritonitis; Adolescent; Adult; Aged; Aged, 80 and over; Enzyme-Linked Immunosorbent Assay; Female; Humans; Interferon-gamma; Male; Middle Aged; Sensitivity and Specificity; Tuberculosis; Young Adultjournal article10.1016/j.jinf.2009.10.001198192582-s2.0-71549131922