Chen S.-HWu C.-CLin S.-CTseng W.-LHuang T.-CYadav ALu F.-ILiu Y.-HSHAU-PING LINHsueh Y.-Y.2022-04-252022-04-25202116616596https://www.scopus.com/inward/record.uri?eid=2-s2.0-85105499728&doi=10.3390%2fijms22094746&partnerID=40&md5=f065be07da336717cda71364b9bc5bbbhttps://scholars.lib.ntu.edu.tw/handle/123456789/606195Peripheral compressive neuropathy causes significant neuropathic pain, muscle weakness and prolong neuroinflammation. Surgical decompression remains the gold standard of treatment but the outcome is suboptimal with a high recurrence rate. From mechanical compression to chemical propagation of the local inflammatory signals, little is known about the distinct neuropathologic patterns and the genetic signatures after nerve decompression. In this study, controllable mechanical constriction forces over rat sciatic nerve induces irreversible sensorimotor dysfunction with sustained local neuroinflammation, even 4 weeks after nerve release. Significant gene upregulations are found in the dorsal root ganglia, regarding inflammatory, proapoptotic and neuropathic pain signals. Genetic profiling of neuroinflammation at the local injured nerve reveals persistent upregulation of multiple genes involving oxysterol metabolism, neuronal apoptosis, and proliferation after nerve release. Further validation of the independent roles of each signal pathway will contribute to molecular therapies for compressive neuropathy in the future. ? 2021 by the authors. Licensee MDPI, Basel, Switzerland.Chronic constriction injuryCompressive neuropathyDecompressionNerve releaseNeuroinflammationNeuropathic painRat sciatic nervecaspase 3CD68 antigencomplementary DNAcytokineinterleukin 1betaisofluranemessenger RNAneuropeptideprotein bcl 2tiletamine plus zolazepamtumor necrosis factorvasoactive intestinal polypeptidexylazineanimal experimentanimal modelanimal tissueArticleaxoncell proliferationchronic constriction injurycontrolled studydecompression surgerydorsal rootgastrocnemius musclegeneral anesthesiaimmunohistochemistrymalemechanical allodyniamuscle atrophymuscle massmuscle weaknessnerve decompressionnervous system inflammationneuroapoptosisneuropathic painneuropathologynonhumanperipheral neuropathypolymerase chain reactionprotein fingerprintingratremyelinizationsciatic nervesciatic nerve injuryspinal ganglionupregulationanimalcrush traumadenervationgene expression profilinggeneticshyperalgesiaimmunologyinflammationinnate immunityinnervationligationneuralgiapathologypostoperative periodsciatic neuropathyskeletal muscleSprague Dawley ratAnimalsAxonsConstrictionCrush InjuriesDecompression, SurgicalDenervationGanglia, SpinalGene Expression ProfilingHyperalgesiaImmunity, InnateInflammationMaleMuscle, SkeletalMuscular AtrophyNeuralgiaPostoperative PeriodRatsRats, Sprague-DawleyRemyelinationSciatic Neuropathyjournal article10.3390/ijms22094746339471042-s2.0-85105499728