Chen C.Ren N.Wang A.Yu Z.Lee D.-J.2019-05-142019-05-14200801757598https://scholars.lib.ntu.edu.tw/handle/123456789/408274High-rate biological conversion of sulfide and nitrate in synthetic wastewater to, respectively, elemental sulfur (S0) and nitrogen-containing gas (such as N2) was achieved in an expanded granular sludge bed (EGSB) reactor. A novel strategy was adopted to first cultivate mature granules using anaerobic sludge as seed sludge in sulfate-laden medium. The cultivated granules were then incubated in sulfide-laden medium to acclimate autotrophic denitrifiers. The incubated granules converted sulfide, nitrate, and acetate simultaneously in the same EGSB reactor to S0, N-containing gases and CO2 at loading rates of 3.0 kg S m -3 d-1, 1.45 kg N m-3 d-1, and 2.77 kg Ac m-1 d-1, respectively, and was not inhibited by sulfide concentrations up to 800 mg l-1. Effects of the C/N ratio on granule performance were identified. The granules cultivated in the sulfide-laden medium have Pseudomonas spp. and Azoarcus sp. presenting the heterotrophs and autotrophs that co-work in the high-rate EGSB-SDD (simultaneous desulfurization and denitrification) reactor. ? 2008 Springer-Verlag.DenitrificationDesulfurizationEGSBGranules[SDGs]SDG6[SDGs]SDG13journal article10.1007/s00253-008-1396-32-s2.0-43349093071https://www.scopus.com/inward/record.uri?eid=2-s2.0-43349093071&doi=10.1007%2fs00253-008-1396-3&partnerID=40&md5=b0007c9d63bbe191fb5d739bd080bb97