2015-11-012024-05-18https://scholars.lib.ntu.edu.tw/handle/123456789/703889摘要：蜘蛛絲是一種具高度特化的結構性材料，可讓蜘蛛用於保護自己與捕捉獵物。由於蜘蛛 絲具有優異的物理特性（機械強度、彈性、可撓曲性、光學度）與生物相容性，故在紡織工 業、軍事材料及醫療方面深具應用之潛力。本計畫係以天然蜘蛛絲纖維為生醫工程材料，以 生物建構(biofabrication)的方式使得蛋白質、酵素或甚至抗體能夠被組裝於蜘蛛絲表面，賦予 蜘蛛絲纖維新的生物化學功能；目前本人已成功地將具生化機能之蜘蛛絲系統用於動物癌症 細胞株之純化與分離，其中包含頭頸部癌細胞株(PCI-15B；高表面biomarker EGFR 表達) 與 大腸癌細胞株(HT29；具高biomarker EpCAM 表達)。本計劃係延續此細胞分離之技術基礎， 計畫把此蜘蛛絲系統應用於生物微流體裝置中，並結合水凝膠(hydrogel)包覆技術，以進行原 位三度空間細胞培養技術(in situ 3D cell cultivation)，預期將來能用來分離與放大具高度復發 與轉移性之血液腫瘤細胞(circulating tumor cells)或癌症幹細胞(cancer stem cells)等，更可進一 步分析被純化之細胞在此三度空間培養系統中之生長或分化過程，預計此平台技術將有利於 後續高通量藥物之篩選技術、臨床分析與基礎研究等。<br> Abstract: As a highly specialized structural material in its native form, spider silk is exploited by the spiders for prey capture and protection purposes. It is of great interests in the fields of textile industry, military, and biomedicine owing to its intriguing mechanical properties (strength, toughness, elasticity, flexibility and optical clarity) and biocompatibility. Aiming to promote the utility of spider silk in biotechnology, I implemented a biofabrication strategy to innovatively endow biochemical functionalities onto the native spider silk, e.g. proteins, enzymes or even antibodies. Such functionalized silk material was then able to execute new tasks with its new features. For instance, the spider silk grafted with antibodies was demonstrated to selectively capture cancer cells of interest, including head and neck cancer cells (PCI-15B; EGFR+) and colon cancer cells (HT29; EpCAM+). In this proposal, I will further integrate the functionalized spider silk into a microfluidic device, creating the environment for in situ capture, encapsulation, and 3D cultivation of target cells. This in situ cell purification and amplification platform will be utilized for the enrichment of rare cancer cells, including circulating tumor cells (CTCs) or cancer stem cells (CSCs). I envision the spider silk based microfluidic system can potentially offer not only an in vivo-mimicking environment to study cancer progression but also a facile platform for advance drug screening and diagnostic technologies蜘蛛絲微流體血液腫瘤細胞癌症幹細胞原位細胞培養Spider silkMicrofluidicsCirculating tumor cells (CTC)Cancer stem cells (CSC)In situ 3D cell cultivation