WOS:000259901000047Hovhannisyan, V.A.V.A.HovhannisyanSu, P.-J.P.-J.SuCHEN-YUAN DONG2019-12-192019-12-19200810833668https://scholars.lib.ntu.edu.tw/handle/123456789/436370https://www.scopus.com/inward/record.uri?eid=2-s2.0-58149386483&doi=10.1117%2f1.2950314&partnerID=40&md5=06829b3099e2e7ae5b7779a28a2dfa8cThe effects of off-axis optical aberration in multiphoton microscopy and the resulting lateral and axial image inhomogeneity are investigated. The lateral inhomogeneity of the scanning field is demonstrated by second harmonic generation (SHG) imaging of fasciae and two-photon fluorescence (TPF) microscopy of thin fluorescent samples. Furthermore, refractive index mismatch-caused intensity attenuation of the TPF signal at central and peripheral regions of the scanning frame is measured using homogeneous 10-μM sulforhodamine B samples with refractive indexes of 1.33 and around 1.465. In addition to characterizing image field convexity, we also found that image resolution degrades away from the optical axis. These effects need to be accounted for in both qualitative and quantitative multphoton imaging applications. © 2008 Society of Photo-Optical Instrumentation Engineers.fluorescence; multiphoton; point spread function; second harmonic generation; spherical aberrationImage fields; Imaging applications; Inhomogeneities; Lateral inhomogeneity; Multi-photon microscopy; Multiphotons; Off-axis; Optical aberration; Optical axis; Point-Spread function; Spherical aberrations; Sulforhodamine B; Two photon fluorescence; Aberrations; Fluorescence; Image resolution; Nonlinear optics; Optical transfer function; Refractive index; Harmonic generation; animal; anisotropy; article; artifact; chicken; computer assisted diagnosis; cytology; evaluation; fascia; image enhancement; methodology; multiphoton microscopy; reproducibility; sensitivity and specificity; Animals; Anisotropy; Artifacts; Chickens; Fascia; Image Enhancement; Image Interpretation, Computer-Assisted; Microscopy, Fluorescence, Multiphoton; Reproducibility of Results; Sensitivity and Specificityjournal article10.1117/1.2950314190213512-s2.0-58149386483https://www.scopus.com/inward/record.uri?eid=2-s2.0-58149386483&doi=10.1117%2f1.2950314&partnerID=40&md5=06829b3099e2e7ae5b7779a28a2dfa8c