Development of an assay system for the analysis of host RISC activity in the presence of a potyvirus RNA silencing suppressor, HC-Pro

Hong, Syuan-FeiSyuan-FeiHongFang, Ru-YingRu-YingFangWei, Wei-LunWei-LunWeiJirawitchalert, SupidchaSupidchaJirawitchalertPan, Zhao-JunZhao-JunPanHung, Yu-LingYu-LingHungPham, Thanh HaThanh HaPhamChiu, Yen-HsinYen-HsinChiuShen, Tang-LongTang-LongShenHuang, Chien-KangChien-KangHuangSHIH-SHUN LIN2023-03-242023-03-242023-01-171743-422Xhttps://scholars.lib.ntu.edu.tw/handle/123456789/629594To investigate the mechanism of RNA silencing suppression, the genetic transformation of viral suppressors of RNA silencing (VSRs) in Arabidopsis integrates ectopic VSR expression at steady state, which overcomes the VSR variations caused by different virus infections or limitations of host range. Moreover, identifying the insertion of the transgenic VSR gene is necessary to establish a model transgenic plant for the functional study of VSR.enAGO1 degradation; HC-pro; In vitro RISC assay; T-DNA insertion[SDGs]SDG2[SDGs]SDG3[SDGs]SDG13Development of an assay system for the analysis of host RISC activity in the presence of a potyvirus RNA silencing suppressor, HC-Projournal article10.1186/s12985-022-01956-2366505052-s2.0-85146406959WOS:000913285900002https://api.elsevier.com/content/abstract/scopus_id/85146406959