Lin H.-P.YI-PING WANGCHUN-PIN CHIANG2021-07-072021-07-0720111043-3074https://www.scopus.com/inward/record.uri?eid=2-s2.0-79951705384&doi=10.1002%2fhed.21452&partnerID=40&md5=fe9f8fa9becdf22f68851a86fcc0ec38https://scholars.lib.ntu.edu.tw/handle/123456789/570299Background Oral verrucous hyperplasia is a precancerous lesion of oral verrucous carcinoma. Methods This study used immunohistochemistry to examine the expression of p53, murine double minute 2 (MDM2), p21, heat shock protein 70 (HSP 70), and human papillomavirus (HPV) 16/18 E6 proteins in 48 oral verrucous carcinoma and 30 oral verrucous hyperplasia samples. Results The mean labeling indices of p53, MDM2, p21, HSP 70, and HPV 16/18 E6 proteins in oral verrucous carcinoma samples were 21%, 31%, 7%, 17%, and 0.5%, respectively, and those in oral verrucous hyperplasia samples were 19%, 35%, 11%, 14%, and 0.3%, respectively. Conclusions Immunohistochemistry with the above-cited 5 biomarkers could not help differentiate oral verrucous hyperplasia from oral verrucous carcinoma. The low expression of p21 may partially explain abnormal epithelial overgrowth in both verrucous lesions. The pathogenesis of both verrucous lesions may be at least partially attributed to the overexpression of MDM2 protein and moderate expression of HSP 70 protein in both lesions. Copyright ? 2009 Wiley Periodicals, Inc.heat shock protein 70; MDM2; p21; p53; verrucous carcinoma; verrucous hyperplasia[SDGs]SDG3heat shock protein 70; protein E6; protein MDM2; protein p21; protein p53; tumor marker; adult; aged; article; carcinogenesis; controlled study; diagnostic test accuracy study; differential diagnosis; epithelioid cell; female; gene overexpression; human; Human papillomavirus type 16; Human papillomavirus type 18; human tissue; hyperplasia; immunohistochemistry; labeling index; major clinical study; male; mouth carcinoma; oral verrucous carcinoma; oral verrucous hyperplasia; pathogenesis; priority journal; protein expression; verrucous carcinoma; Adolescent; Adult; Aged; Aged, 80 and over; Carcinoma, Verrucous; Child; Cohort Studies; DNA, Viral; Female; Gene Expression Regulation, Neoplastic; HSP70 Heat-Shock Proteins; Human papillomavirus 16; Human papillomavirus 18; Humans; Hyperplasia; Immunohistochemistry; Male; Middle Aged; Mouth Diseases; Mouth Neoplasms; p21-Activated Kinases; Precancerous Conditions; Proto-Oncogene Proteins c-mdm2; Sensitivity and Specificity; Tumor Markers, Biological; Tumor Suppressor Protein p53; Young Adultjournal article10.1002/hed.21452205831152-s2.0-79951705384