伍安怡2018-07-092018-07-092004http://ntur.lib.ntu.edu.tw//handle/246246/25371To investigate the immunoapthogenesis of SARS, we infected a number of human cell lines with SARS-CoV. By immunoflourescent staining with sera from SARS patients, we identified one monocytic cell line that is susceptible to the virus. Total RNA was extracted from the cells to assay for the expression of chemokines. Results of Multi-Probe RNase protection assay demonstrated that the monocytic cell line expressed a panel of chemokines after SARS-CoV infection, while the epithelial cell line expressed only one. In addition, SARS-CoV-infected epithelial cell expressed adhesion molecules. Western blot analysis showed that the monocytic as well as the epithelial cells express ACE-2, a putative SARS-CoV receptor. Comparing DC-SIGN transfected cells to their parental cell line; we demonstrated that expression of DC-SIGN did not change the kinetics of chemokines induced by SARS-CoV. Based on our data, we concluded that both human epithelial cells and monocytic cells are hosts for SARS-CoV. The possible scenario of severe acute respiratory distress that occurs in patients infected by SARS-CoV is as follows :( 1) SARS-CoV infects pulmonary epithelial cells; (2) The infected epithelial cells express adhesion molecules and produce chemokine to attract monocytes; (3) The recruited monocytes in turn are infected by SARS-CoV to produce a panel of chemokines; (4) These chemokines are important in recruiting T cells, neutrophils and more monocytes into the lungs, which result in tissue damage and eventual respiratory distress.application/pdf60352 bytesapplication/pdfzh-TW國立臺灣大學醫學院免疫學研究所journal articlehttp://ntur.lib.ntu.edu.tw/bitstream/246246/25371/1/923112B002039.pdf