Ma L.-C.CHI-TAI FANGLee, Cha-ZeCha-ZeLeeCHIA-TUNG SHUNJIN-TOWN WANG2021-04-202021-04-2020050022-1899https://www.scopus.com/inward/record.uri?eid=2-s2.0-20844461347&doi=10.1086%2f430619&partnerID=40&md5=7333bf9720133e7104ad1e3848af1436https://scholars.lib.ntu.edu.tw/handle/123456789/556823Background. Primary pyogenic liver abscess (PLA) with septic complication by Klebsiella pneumoniae is an emerging infectious disease. Methods and results. Using DNA microarray hybridization, we identified a 20-kb chromosomal region that contained 15 open-reading frames (ORFs), including an iron-uptake system (kfu), a phosphoenolpyruvate sugar phosphotransferase system (PTS), and 6 unknown ORFs. The region was more prevalent among tissue-invasive strains (35/46) than among noninvasive strains (19/98) (P < .0001, χ2 test). To test the role played by this region in pathogenesis, 3 different deletion mutants (NTUH-K2044 [Δkfu], K2044 [ΔORF7-9], and K2044 [ΔPTS]) were constructed. Only the ΔkfuABC mutants showed decreased virulence in mice, compared with the wild-type strain. An in vitro assay confirmed the involvement of kfu in iron acquisition. There was a high correlation rate (85%) between the kfu/PTS region and 2 tissue invasion-associated chromosomal regions (allS and magA). Moreover, all 3 regions were present in strains that caused PLA plus endophthalmitis or meningitis. Conclusion. Our results suggest that chromosomal heterogeneity is present in tissue-invasive K. pneumoniae strains. A genotype containing all 3 regions is strongly associated with PLA and metastatic infection. These regions may serve as convenient markers for the rapid diagnosis of emergent tissue-invasive strains. ? 2005 by the Infectious Diseases Society of America. All rights reserved.[SDGs]SDG3amino acid sequence; animal experiment; animal model; animal tissue; article; bacterial chromosome; bacterial meningitis; bacterial strain; bacterial virulence; colorimetry; controlled study; deletion mutant; DNA hybridization; DNA microarray; endophthalmitis; genetic heterogeneity; genotype; Gram negative sepsis; histopathology; human; in vitro study; iron transport; Klebsiella pneumoniae; LD 50; liver abscess; mouse; nonhuman; nucleotide sequence; open reading frame; operon; plasmid; polymerase chain reaction; priority journal; promoter region; slot blot hybridization; strain difference; survival rate; wild type; Animals; Bacterial Proteins; Chromosome Mapping; Endophthalmitis; Genome, Bacterial; Humans; Klebsiella Infections; Klebsiella pneumoniae; Liver Abscess, Pyogenic; Meningitis, Bacterial; Mice; Mice, Inbred BALB C; Microarray Analysis; Plasmids; Sepsis; Variation (Genetics); Virulencejournal article10.1086/430619159429012-s2.0-20844461347