臺灣大學: 化學工程學研究所蔡偉博蔡之琦Tsai, Chih-ChiChih-ChiTsai2013-03-272018-06-282013-03-272018-06-282010http://ntur.lib.ntu.edu.tw//handle/246246/252356Nonfouling的表面由於能夠抵抗蛋白質的吸附，進而抑制細胞或血小板的貼附，所以被廣泛運用在生物材料上。本研究使用具有兩性離子的磺基甜菜鹼單體(sulfobetaine mathacrylate)，利用自由基聚合法合成帶有羧基的化合物(P(SBMA)-COOH)，藉由EDC/NHS的反應接枝於聚電解質修飾的表面(PEI/PAA-AZ/PEI)，達到抗生物性貼附。本研究的目的在於使用這個簡單容易的接枝方法修飾磺基甜菜鹼在不同的表面上，並抑制細胞貼附。 本研究使用不同錬長的磺基甜菜鹼，接枝時的pH值跟反應濃度的影響，來探討細胞貼附的結果。分析表面元素組成和親疏水性，結果顯示，以不同錬長接枝在表面，發現在表面元素和親疏水上並無顯著差異；而對於pH 值的影響，在pH 3和6.5的接枝環境下，表面較未改質之前更為親水，並含有硫的存在，反之 ，在pH 11的接枝環境下，改質前後並無顯著差異。對於細胞貼附與蛋白質吸附實驗結果，pH 3 和6.5的接枝環境下產生的表面，都能抑制蛋白質吸附與細胞和血小板貼附，而pH 11則沒有抑制的效果。除此之外，我們的表面改質方法不僅使用在TCPS基材上，還使用在聚二甲基矽氧烷、聚碸和聚丙烯這三種基材上。顯示此方法可以廣泛運用在多種基材上，並可以達到生物惰性。 我們藉由甜菜鹼單體(sulfobetaine mathacrylate)和含有硫醇官能基的單體，製備共聚物(p(SBMA-SH)-COOH)，並接枝上細胞貼附的氨基酸序列(RGD)，得到一個可調控的表面。對於細胞貼附的結果，接枝RGD後，細胞貼附量並沒有增加。 本研究的結果顯示，藉由我們的改質方式，可以有效的接枝磺基甜菜鹼在表面上而達到抗生物性貼附，並廣泛的運用在不同生醫材料上面。Nonfouling surface has been widely used in biomaterials due to its potential resistancy in non-specific adsorption. In our study, Sulfobetaine methacrylate (SBMA), with its zwitterionic character, was used to fabricate bioinert surfaces. P(SBMA)-COOH was fabricated by free radical polymerization, then it was grafted on PEI/PAA-AZ/PEI polyelectrolyte multilayer by carbodiimide reaction. Through this method, the surface modification of p(SBMA)-COOH can be easily prepared, and the use of polyelectrolytic method can also be applied onto various of substrates to inhibit cell adhesion. Examinations were done on the p(SMBA)-COOH by altering its chain length, grafting pH value and p(SBMA)-COOH grafting concentration. The surface composition and the surface hydrophobicity were measured. As result, the surface became hydrophilic after grafting p(SBMA)-COOH at pH 3 and 6.5, with high content of sulfur. In contrast, there was no significant difference upon its hydrophobicity and content of sufler, before and after p(SBMA)-COOH grafting at pH 11 condition. In addition, pH 3 and 6.5 films exhibited resistance to protein adsorption, cell and platelets adhesion. Different substrates such as PDMS, PSF and PP were also used in fabricating similar modified films, and the cell adhesion resistance were observed on these p(SBMA)-modified substrates. Since RGD-modified surfaces enhance cell adhesion, p(SBMA-SH)-COOH was fabricated by SBMA and 2-propene-l-thiol, and then grafted with RGD peptide for further analysis. As result, the cell density on RGD-modified surface was low. These studies support the potential of our method for p(SBMA)-modified surface fabrication in order to obtain cell resistance. In addition, our method can also be applied on wide range of different substrates.9806065 bytesapplication/pdfen-US生物惰性sulfobetaineRGD細胞貼附血小板貼附蛋白質吸附bioinertsulfobetaine methacrylatecell adhesionplatelet adhesionprotein adsorptionthesishttp://ntur.lib.ntu.edu.tw/bitstream/246246/252356/1/ntu-99-R97524082-1.pdf