2006-03-312024-05-17https://scholars.lib.ntu.edu.tw/handle/123456789/684201摘要：蘭花為台灣重要之經濟作物，具備各種消費者喜好花形、花色之重要性狀,然而許 多負責蘭花重要性狀之基因並未被充分了解及利用。本計畫擬以轉位子建立蘭花 之有用基因釣取(gene tagging/trapping)系統，有助於篩選出蘭花之有用基因及其啟動子，將來並可應用於產業快速建立蘭花之新品種。申請人先前曾建構「可誘導轉位子」成功應用於水稻、蕃茄及菸草等作物。然而，最初之「可誘導轉位子」僅適用於基因篩選，需大量製造同源突變體。據此，申請人先前完成一新構築：將一無啟動子之報導基因（promoter-less reporter gene）建構於先前之「可誘導轉位子」中，新構築轉入作物則不需製造同源突變體即可開始篩選有用基因，該構築已轉入農桿菌並開始執行轉基因蝴蝶蘭工作。本年度計畫將延續先前工作，將一超強啟動子建構於先前之「可誘導轉位子」中並轉入蝴蝶蘭，執行activation tagging開發蘭花新品種。 <br> Abstract: Orchid is an important industry in Taiwan. In order to sustain and expand the market,genetic engineering has been recognized as an approach to overcome the limitations ofimprovement through conventional breeding. Through genetic engineering, genes for shelf life, flower color and architecture can be directly manipulated to develop new varieties that are tailor made to customers' preferences. However, many Phalaenopsis genes remained to be cloned and engineered. In this study, we will use the inducible transposon to clone the useful genes of Phalaenopsis and furthermore study their function. Transposon has been demonstrated to be a powerful tool for cloning plant genes. Thus, we have constructed an inducible transposon (INAc) and demonstrated that it is functional in rice, tomato and tobacco plants. However, by using the INAc, most plant genes could be cloned only by creating homozygous mutants. This feature limits the application of INAc for cloning genes from many important plants, e.g. Phalaenopsis. Thus, we have constructed a new inducible transposon (termed as INAcGFP) containing a promoter-less reporter gene (GFP). This allows us to clone the useful gene from T0 generation of transgenic Phalaenopsis plant. In this project, a 4x 35S promoter would be constructed into the inducible transposon to perform activation tagging. The mutant screening could be started since after the successful transformed Phalaenopsis are produced.可誘導轉位子轉基因蝴蝶蘭inducible transposontransgenic Phalaenopsis