嫁接與春化作用對甘藍開花之影響及其分子調控機制

2016-08-012024-05-18https://scholars.lib.ntu.edu.tw/handle/123456789/711776摘要：甘藍為綠植株春化型(plant-vernalization-responsive)植物，因此在很少出現足夠低溫的熱帶與亞熱帶平地地區需低溫處理(春化作用)來誘導開花及生產種子。此外，已知嫁接(grafting)技術廣泛用於葫蘆科及茄科蔬菜商業生產，以便增加產量、增強對土壤傳染性病害及非生物性逆境的抗性、提高生長勢、及誘導或促進開花等。由於春化與嫁接皆可誘導或促進植株開花，本試驗希望建立甘藍嫁接技術、探討嫁接與春化作用對甘藍開花之影響，並藉此研究甘藍開花分子調控機制。因此，本計畫內容包含有(一)研究根砧種類與品種對甘藍嫁接苗開花之影響；(二)建立甘藍嫁接癒合條件；(三)研究春化作用對甘藍嫁接苗開花之影響；(四)建立甘藍嫁接方式及(五)研究與嫁接和春化作用相關的甘藍開花分子調控機制。上述研發之技術將可應用於臺灣或其他亞熱帶平地地區甘藍之採種及育種工作。<br> Abstract: Cabbage is a plant-vernalization-responsive plant, thus requiring low temperature treatment (vernalization) to induce flowering and seed production in tropical and subtropical lowland areas where sufficient low temperature seldom occurs. In addition, it is known that grafting technique is widely used in commercial production of cucurbits and solanaceous vegetables, so as to, e.g., increase yield, improve resistance to soil-borne diseases and abiotic stresses, promote plant vigor, and induce or promote flowering. Given that vernalization and grafting both can induce or promote plant flowering, this research aims to develop cabbage grafting technique, investigate effects of grafting and vernalization on cabbage flowering, and discuss the molecular mechanisms of cabbage flowering regulation. Accordingly, the contents of this research will include (1) Studying the effects of rootstock species and varieties on flowering of grafted cabbage, (2) Setting up the acclimatization conditions for cabbage grafting, (3) Studying the effects of vernalization on flowering of grafted cabbage, (4) Developing the cabbage grafting method, and (5) Studying the molecular mechanisms of cabbage flowering regulation associated with grafting and vernalization. The techniques developed accordingly will be useful for seed production and breeding of cabbage in the lowland areas in Taiwan and other subtropical zones.甘&#63779嫁接開花春化作用cabbagegraftingfloweringvernalization嫁接與春化作用對甘藍開花之影響及其分子調控機制