2011-01-012024-05-14https://scholars.lib.ntu.edu.tw/handle/123456789/658339摘要：全球氣候溫暖化的趨勢，使得台灣遭受嚴重的蚊蟲孳擾與蚊媒傳染病威脅。長期密集使用化學藥劑來毒殺蚊蟲已經使得蚊蟲產生嚴重的抗藥性。本研究主要利用台灣本土的橈足類劍水蚤來進行蚊幼蟲防治，以發展一個兼具簡單、作用專一性高、蚊蟲不會產生抗藥性、可節省噴藥費用和大量人力等優點的新型式生物性誘卵滅幼方法，以達到病媒蚊族群控制的終極目標。本研究規劃為三年期。第一年，調查南台灣嘉南高高屏地區的橈足類劍水蚤種類，分析哪些劍水蚤具有捕食蚊蟲的能力和較高的捕食效力，利用分子生物學方法分析是否攜帶未知病原微生物，並分析劍水蚤對現行殺蟲藥劑之感受性，最後於實驗室建立劍水蚤人工培養的系統，以提供第二年之田間試驗分析使用。第二年，透過地理資訊系統分析劍水蚤之分布地點、病媒蚊聚集區、歷年本土登革陽性個案或群聚資料，規劃評估以橈足類劍水蚤為主的生物性誘卵滅幼器之實務應用分析方法，以及建立定點中大型容器及地下室積水之監測站，透過試驗設計分析了解劍水蚤對登革病媒幼蟲之防治效力。第三年，持續追蹤以橈足類劍水蚤為主的誘卵滅幼器的系統、定點中大型容器及地下室積水之監測站之生態功效，以達到病媒長期監測和蚊蟲控制的雙重功效。本研究內容包含基礎生物學特性、研究室驗設計分析和實務應用的可行性評估，這種生態友善的生物防制方法，長期施行不僅能達成病媒管制的目的，降低疾病傳播的風險，還能兼顧生物多樣性而達到環境永續的精神。<br> Abstract: With the trend of global warming, mosquito-borne infectious diseases have not only being an issue but also a serious impact in Taiwan. Using chemical pesticide intensively has raised serious problem of insect resistance. The goal of this study is to control mosquito populations by establishing mosquito control strategies with benefits of eco-friendly, sustainable, simple, highly specificity, without chemical pesticide, low cost and low human labor. Therefore, we evaluated the Taiwanese freshwater cyclopid copepods that may fit to traditional ovitrap and can be used as an integrated biological vector control strategy. Scheduled process to study and evaluate this strategy will contain three years. In the first year, field surveillance of freshwater cyclopid copepods will be carried out in Tainan, Kaohsiung and Pingtung in southern Taiwan, where are dengue hot spots with indigenous dengue or with highly mosquito index. Specific species with the ability and higher efficiency to prey mosquito larvae will be selected and then artificial-cultured in laboratory for the further study in the next year. Molecular diagnosis of unknown pathogenic microbes vectored by copepods and their sensitivity to cutrrent used pestiside will be further analyzed. In the second year, scheduled field evaluation experiments will be carried out based on the geographic information system (GIS) and clustering cases of indigenous dengue fever. This study will set up sentinel spots, such as biger artificial containers and flooding-basement, for long-term ecological surveillance. In the last year, evaluation and application of these biological agents incorporated to the ovitrap will be carried out to optimize the biological functions on mosquito surveillance and vector control. Over all, this study contains basic research of mosquito biology and microbiology, experimental design, and field applications. These kinds of ecological friendly approached bio-control strategies will lead to environmental sustainable. Goals achievement in this study revealed not only successes in vector control but also biological diversity.橈足類劍水蚤誘卵器登革熱病媒蚊誘卵滅幼器體腔真菌生態友善CyclopidCopepodsovitrapDengue fevervectorovi-larvicidal trapCoelomomycesEco-friendly