2011-01-012024-05-13https://scholars.lib.ntu.edu.tw/handle/123456789/650362Purpose Pancreatic cancer is one of the most aggressive cancers. In Taiwan, pancreatic cancer is the tenth leading cause of cancer-related death, and the incidence has been increasing gradually in recent years. Because of its safety and reliability, endoscopic ultrasound-guided fine needle aspiration (EUS-FNA) is currently becoming the standard diagnostic procedure for pancreatic cancer. On-site cytology evaluation of EUS-FNA specimens is useful for quality assurance with improved diagnostic yield. The objective of this study was to evaluate whether Ultrafast Papanicolaou (UFP) stain with 90-second protocol could be helpful in EUS-FNA on-site cytology. Methods We adopted UFP stain with Hematoxylin 2 and Cyto-stain for on-site cytology assessment along with Hemacolor stain. Results One hundred and seventeen consecutive patients of pancreatic tumors were enrolled in this study. The individual performance characters were as follows, sensitivity 98.9%, specificity 100%, positive predictive value 100%, negative predictive value 96.2% and accuracy 99.1%. These figures showed improvement in accuracy after the use of UFP. On-site cytology evaluation with UFP stain could offer transparency with polychromasia as traditional Papanicolaou stain, and presented with better nuclear details than Hemacolor stain. UFP stains would be helpful in pancreatic tumors with distinctive nuclear pattern such as pancreatic endocrine tumor and hypervascularity. Conclusion In this study, we found UFP to be a good adjunctive stain for on-site evaluation during EUS-FNA of pancreatic tumors with high accuracy. UFP stain has offered better nuclear features and cleaner background. Combination of UFP stain and rapid Romanowsky-type stain may aid in rapid EUS-FNA cytology diagnosis with good accuracy.Ultrafast Papanicolaou StainPancreatic Tumors