2010-01-012024-05-17https://scholars.lib.ntu.edu.tw/handle/123456789/682431摘要：本年度目標乃是觀測免克寧於土壤中的降解情形，並探討探討其對微生物菌相的影響，最終期望能實際解決免克寧在環境中的污染情形。解免克寧於環境中的降解情形，做為日後對環境中免克寧之清除依據。觀測免克寧對環境中微生物的影響，藉以評估免克寧對環境之衝擊。<br> Abstract: Vinclozolin has found to have the anti-androgen potential, and its metabolites, M1 and M2, are currently found to have more affinity to androgen receptors than their parent chemical, vinclozolin. Also, its final metabolite, 3,5-dichloroaniline, is found for more acute nephrotoxicity than other dichloroaniline stereoisomers. To monitor the degradation of vinclozolin and its metabolites production, and to investigate the impact of vinclozolin with its metabolites on soil microbial community, we used DNA extracted kits to collect the soil microbe’s DNA, and used solvent phase extraction to extract vinclozolin and its metabolites. Then we utilized HPLC-UV and GCECD to detect vinclozolin and metabolites in soil sample. The influences of vinclozolin and its metabolites on soil microbial community were visualized by PCR-DGGE.免克寧微生物菌相降解Vinclozolinmicrobial communitydegradation