2006-03-012024-05-17https://scholars.lib.ntu.edu.tw/handle/123456789/682388摘要：中文摘要： E型肝炎是經口糞感染的非A及非B型肝炎，在開發中國家因水源遭受污染常有爆發性流行，為一重要的公共衛生疾病。E型肝炎感染者一般死亡率約在1%左右，但在孕婦，尤其第三期妊娠感染者，死亡率可達20%。E型肝炎病毒在豬隻身上亦被發現，在日本曾有因直接生食野鹿肉或野豬肝臟而導致發病死亡的病例；此病原在豬隻飼養、屠宰及獸醫師等相關人員有明顯高血清抗體陽性率。台灣豬E型肝炎病毒感染相關情形雖有部分報告，但相關的研究及疫情掌握仍有不足，為因應未來此一人畜共通傳染病及畜產品產製環境之人畜安全檢測需要，本計劃擬建立及開發豬E型肝炎病毒之快速診斷技術及試劑。 <br> Abstract: Hepatitis E is a non-A, non-B hepatitis and is transmitted by the fecal-oral route. Contamination of drinking water has become the main reason to cause outbreaks of hepatitis E in several developing countries. It is an important disease with public health concern. The mortality rate of hepatitis E is generally low, about 1% only; however, it can be more serious in pregnant woman, especially during the third trimester, reaching 20%. Strains of hepatitis E virus have also been detected in pigs. In Japan, people were infected with hepatitis E virus and resulted in death after consuming raw wild boar liver and raw deer meet. Significantly higher serum-positive rate was also seen in pig handlers, including pig producer, pig slaughter worker, and veterinarian. Although there have been some papers showing the domestic condition of hepatitis E virus infection in pigs, the information is still limited. For the future needs of diagnosing this zoonotic disease and preventing its spreading between man and animals, the objectives of the present study are to establish and develop rapid diagnostic methodologies and reagents for the detection of swine hepatitis E virus.豬E型肝炎病毒反轉錄聚合&#37238鏈鎖反應原位雜交swine hepatitis E virusRT-PCRin situ hybridization