2015-01-012024-05-14https://scholars.lib.ntu.edu.tw/handle/123456789/659270摘要：收集臺灣之養殖九孔&#30129;疹病毒，純化分析九孔&#30129;疹病毒之基因系列，並完成&#30129;疹病 毒II型之引子對篩選,及部分基因系列分析並和I型病毒比對。應用於病材之快速診 斷，縮短檢查所需時間並可在現場應用，因此可及早偵測水產生物是否感染&#30129;疹病 毒，避免引入帶病毒之魚種，減少本病爆發及疫情擴散。建立篩選建立九孔重要病 毒性疾病之檢驗方法，並據此應用在檢疫用。研發病毒檢測法，建立新疫病資料 ，以利輸出入水產動物的檢疫工作。持續研究九孔&#30129;疹病毒，包括病毒特性、致病 機制及疫病控制的相關研究。 <br> Abstract: Abalone herpes virus has been reported to infection cultured abalone in Taiwan and Australia in the past years. The diagnosis of this disease by PCR and real time PCR has been developed. However, these methods are time consuming, laborious and sometimes non-specific. Loop-mediated isothermal amplification is a rapid, sensitive, inexpensive and powerful tool in applying in different pathogens including fish and shellfish pathogens such as White spot syndrome virus, Taura syndrome virus, Flavobacterium columnare, Spring viraemia of carp virus and Cyprinid herpesvirus. In this study, this technique will be study to develop a specific diagnostic protocol for detection of abalone herpes virus for quarantine purpose. 3九孔&#30129疹病毒檢疫蛋白質表現abaloneherpesvirusquarantineprotein expression