Ke, Meng-WeiMeng-WeiKeJiang, Yan-NianYan-NianJiangLi, Yi-HungYi-HungLiTseng, Ting-YuTing-YuTsengKung, Ming-ShungMing-ShungKungCHIUN-SHENG HUANGCheng, Winston Teng-KueiWinston Teng-KueiChengHsu, Jih-TayJih-TayHsuJu, Yu-TenYu-TenJu2012-01-302018-06-292012-01-302018-06-2920071011-2367http://www.scopus.com/inward/record.url?eid=2-s2.0-34249911216&partnerID=MN8TOARShttp://scholars.lib.ntu.edu.tw/handle/123456789/329085http://ntur.lib.ntu.edu.tw/bitstream/246246/238490/-1/05.pdfCaveolin-1 of the caveolin family of proteins regulates mammary gland development and has been shown to play a contradictory role in breast tumor progression. A specific anti-Caveolin-1 antibody will be useful for functional study of Caveolin-1 in different tissues. In this study, we generated a rabbit polyclonal antibody that specifically recognizes the N-terminal amino acids 50-65 of Caveolin-1. This polyclonal antibody specifically reacted with Caveolin-1 extracted from cells of different species, including human epithelial A431 cells, goat primary mammary epithelial cells and mice fibroblast NIH 3T3 cells, by Western blotting. Endogenous Caveolin-l protein expressing in cells and normal human tissues was detected by this polyclonal antibody using immunocytofluorescent and immunohistochemical staining, respectively. Furthermore, an apparent decrease in Caveolin-1 expression in tumorous breast and colon tissues was detected by this polyclonal antibody. In conclusion, we have identified amino acids 50-65 of Caveolin-1, which contains an epitope that is specific to Caveolin-l and is conserved in the human, goat and mouse. In future, this anti-Caveolin-1 antibody can be used to examine the progression of breast and colon cancers and to study functions of Caveolin-1 in human, goat and mouse cells.[SDGs]SDG3Capra hircus; Mus; Oryctolagus cuniculusjournal article10.5713/ajas.2007.8562-s2.0-34249911216http://ntur.lib.ntu.edu.tw/bitstream/246246/238490/-1/05.pdf