Wu, Ming-FangMing-FangWuYang, Chih-YaChih-YaYangLin, Tzu-LungTzu-LungLinJIN-TOWN WANGYang, Feng-LingFeng-LingYangWu, Shih-HsiungShih-HsiungWuHu, Bor-ShenBor-ShenHuChou, Teh-YingTeh-YingChouTsai, Ming-DawMing-DawTsaiLin, Chi-HungChi-HungLinHsieh, Shie-LiangShie-LiangHsieh2009-07-292018-07-062009-07-292018-07-062009http://ntur.lib.ntu.edu.tw//handle/246246/162876Klebsiella pneumoniae magA (for mucoviscosity-associated gene A) is linked to the pathogenesis of primary pyogenic liver abscess, but the underlying mechanism by which magA increases pathogenicity is not well elucidated. In this study, we investigated the role of the capsular polysaccharides (CPS) in the pathogenesis of magA+ K. pneumoniae by comparing host immunity to magA+ K. pneumoniae and a ΔmagA mutant. We found that Toll-like receptor 4 recognition by magA+ K pneumoniae was hampered by the mucoviscosity of the magA + K. pneumoniae CPS. Interestingly, monoclonal antibodies (MAbs) against magA+ K pneumoniae CPS recognized all of the Kl strains tested but not the ΔmagA and non-Kl strains. Moreover, the anti-CPS MAbs protected mice from magA+ K. pneumoniae-induced liver abscess formation and lethality. This indicates that the Kl epitope is a promising target for vaccine development, and anti-CPS MAbs has great potential to protect host from Kl strain-induced mortality and morbidity in diabetic and other immunocompromised patients in the future. Copyright ? 2009, American Society for Microbiology. All Rights Reserved.application/pdf1635553 bytesapplication/pdfen-US[SDGs]SDG3epitope; monoclonal antibody; polysaccharide; toll like receptor 4; bacterial polysaccharide; bacterial protein; MagA protein, Klebsiella pneumoniae; monoclonal antibody; Tlr4 protein, mouse; toll like receptor 4; article; bacterial gene; bacterial membrane; bacterial strain; controlled study; human; human cell; humoral immunity; Klebsiella pneumoniae; lethality; liver abscess; magA gene; molecular recognition; morbidity; mortality; mouse; mutant; nonhuman; pathogenesis; priority journal; signal transduction; animal; antibody production; bacterial membrane; Bagg albino mouse; cell line; chemistry; gene expression regulation; genetics; heat; immunology; Klebsiella infection; Klebsiella pneumoniae; macrophage; metabolism; microbiology; mutation; physiology; Animals; Antibodies, Monoclonal; Antibody Formation; Bacterial Capsules; Bacterial Proteins; Cell Line; Gene Expression Regulation, Bacterial; Hot Temperature; Humans; Klebsiella Infections; Klebsiella pneumoniae; Macrophages; Mice; Mice, Inbred BALB C; Mutation; Polysaccharides, Bacterial; Signal Transduction; Toll-Like Receptor 410.1128/IAI.00931-08http://ntur.lib.ntu.edu.tw/bitstream/246246/162876/1/06.pdf