Yang, K.-C.K.-C.YangWu, C.-C.C.-C.WuCheng, Y.-H.Y.-H.ChengKuo, T.-F.T.-F.KuoTZONG-FU KUOFENG-HUEI LIN2020-02-262020-02-262008https://scholars.lib.ntu.edu.tw/handle/123456789/464033Purpose: A chitosan/gelatin solution with glycerol 2-phosphate disodium salt hydrate in liquid phase at room temperature becomes a hydrogel at 37°C. The material can be used as an injectable cell carrier into the human body for gelation in situ. We hoped that the chitosan/gelatin hydrogel provided extra protection for insulinoma/agarose microspheres during xenogenic transplantation. Materials and Methods: Mouse insulinoma was microencapsulated in agarose as microspheres, which were macroencapsulated in chitosan/gelatin hydrogel. Insulin secreting profiles were first demonstrated in vitro. Diabetic rats were injected subcutaneously with insulinoma/agarose microspheres or insulinoma/agarose microspheres suspended in chitosan/gelatin solution. The nonfasting blood glucose concentrations (NFBG) of diabetic rats were measured perioperatively. Rats were humanely killed 1 month postoperatively and the hydrogel was retrieved for histological examination. Results: The insulinoma/agarose microspheres continually secreted insulin for 1 month when macroencapsulated in chitosan/gelatin hydrogel in vitro. The NFBG of diabetic rats injected with insulinoma/agarose microspheres decreased to euglycemic status albeit hyperglycemia was restored within 10 days. The NFBG of diabetic rats injected with chitosan/gelatin hydrogel, which contained insulinoma/agarose microspheres, was maintained at less than 200 mg/dL for 25 days. The histological section revealed immune cell infiltration and accumulation within the hydrogel and around the iusulinoma/agarose microspheres that may have contributed to the slowly increasing NFBG after day 25. Conclusion: This study showed that chitosan/gelatin hydrogel can be used as a cell carrier for an injectable bioartificial pancreas; the hydrogel prolonged the function of cells encapsulated in agarose microspheres during xenogenic transplantation. ? 2008 Elsevier Inc. All rights reserved.[SDGs]SDG3agarose; chitosan; gelatin; glucose; glycerol 2 phosphate; microsphere; animal cell; animal experiment; animal model; animal tissue; article; cell infiltration; concentration (parameters); diabetes mellitus; gelation; glucose blood level; hydrogel; hyperglycemia; immunocompetent cell; in vitro study; in vivo study; insulin release; insulinoma; liquid; microencapsulation; mouse; nonhuman; priority journal; protection; rat; room temperature; xenograft; xenotransplantation; Animals; Chitosan; Gelatin; Hydrogels; Insulin; Insulinoma; Mice; Neoplasm Transplantation; Rats; Sepharose; Transplantation, Heterologousjournal article10.1016/j.transproceed.2008.06.0922-s2.0-57549116863https://www.scopus.com/inward/record.uri?eid=2-s2.0-57549116863&doi=10.1016%2fj.transproceed.2008.06.092&partnerID=40&md5=e2e09f2694b4860d6cdf93038fa87707