2011-03-182024-05-14https://scholars.lib.ntu.edu.tw/handle/123456789/660611摘要： 本計畫目標是製備適於偵測生物分子及調控酵素活性之適合體(aptamer)修飾的金奈米粒子( gold nanoparticles; Au NPs)。相較於傳統抗體，適合體有易製備、易修飾、易保存、分子量低和低成本等優點。為克服蛋白質或分子適合體與目標分子的弱結合力問題，我們將其修飾於奈米粒子上，藉由多重結合(multivalent binding)效應和/或粒子表面高區域濃度(local concentration)來增強其與目標酵素的親合力。修飾於奈米粒子表面之適合體的穩定度高，故可應用於實際生物樣品或細胞之研究。本計畫將提出奈米粒子表面自排列/組裝(self-assembled arranged monolayer, SAAM)的技術：將單股DNA修飾於奈米粒表面與另一單股DNA延伸之適合體於奈米粒子表面互補後，即可製備功能性SAAM奈米粒子。藉由功能性奈米粒子與酵素（凝血酶；thrombin）結合後，光學或催化活性的改變來達偵測目的。另外這些功能性奈米粒子亦可應用於調控酵素活性。<br> Abstract: Our project goal is to prepare aptamers conjugated gold nanoparticles (Apt-Au NPs) for the detection of proteins and for the control of the activity of enzymes. Relative to conventional antibodies, aptamers provides several advantages, including ease in preparation and modification, stability, low molecular weight, and low cost. To provide high selectivity, we propose self-assembled arranged monolayer (SAAM) approaches to the preparation of functionalized Au NPs. Through multivalent binding and high local concentration, the functionalized Au NPs can provide high selectivity toward the target analytes. To prepare such functionalized Au NPs, one type of DNA molecules will be conjugated to Au NP surfaces and the functionalized DNA molecules will be attached to the DNA molecules through hybridization. Our sensing mechanisms will be based on analytes (thrombin) induced changes in the DNA conformation, leading to changes in the optical properties of the Au NPs or the catalytic activity of thrombin.適合體金奈米粒子奈米粒子表面自排列/組裝凝血？aptamergold nanoparticlesAu NPsself-assembled arranged monolayer, SAAMthrombin