Ho S.-H.Xie Y.Chan M.-C.Liu C.-C.Chen C.-Y.Lee D.-J.Huang C.-C.Chang J.-S.2019-05-142019-05-14201509608524https://scholars.lib.ntu.edu.tw/handle/123456789/408322In this study, the effects of the type and concentration of nitrogen sources on the cell growth and lutein content of an isolated microalga Scenedesmus obliquus FSP-3 were investigated. With batch culture, the highest lutein content (4.61. mg/g) and lutein productivity (4.35. mg/L/day) were obtained when using 8.0. mM calcium nitrate as the nitrogen source. With this best nitrogen source condition, the microalgae cultivation was performed using two bioreactor strategies (namely, semi-continuous and two-stage operations) to further enhance the lutein content and productivity. Using semi-continuous operation with a 10% medium replacement ratio could obtain the highest biomass productivity (1304.8. mg/L/day) and lutein productivity (6.01. mg/L/day). This performance is better than most related studies. ? 2014 Elsevier Ltd.Bioreactor strategyLight intensity shiftLuteinMicroalgaeScenedesmus obliquusjournal article10.1016/j.biortech.2014.10.0622-s2.0-84939957365https://www.scopus.com/inward/record.uri?eid=2-s2.0-84939957365&doi=10.1016%2fj.biortech.2014.10.062&partnerID=40&md5=5ab8e22950ef7b8320311dfbb550ff84