2019-01-162024-05-17https://scholars.lib.ntu.edu.tw/handle/123456789/683925摘要：流感病毒是每年造成老人、兒童肺炎的主要原因之一，此外也會引起中耳炎、急性支氣管炎、腦炎等症狀。由於流感病毒基因變異性大，它在世界各地常造成每年的大流行，使得許多人因此罹病、甚至死亡，對社會造成相當大的衝擊。流感疫苗是預防流感最有效之策略，國際間的實證皆支持健康個人接種流感疫苗可獲得保護力，降低感染流感之嚴重度或死亡，惟實際的保護效果，仍需透過血清流行病學的調查來確認。計畫目的為藉由實驗室檢測流感病毒血清流行病學調查，配合相關人口學資料並對照其流感疫苗接種紀錄，瞭解其對流感病毒之保護力，作為未來預測流行病毒、疫情規模及疫苗接種等政策之參考依據。本實驗室將於每年8月至11月收案共60名，對象為健康且年紀介於2歲至6歲之幼童，收集血液檢體及進行問卷調查。利用HAI assay(血液凝集抑制方法)檢測體內流感抗體，針對H1N1、H3N2、 B/Vitoria、 B/Yamagata，此四型本土流行之流感病毒，瞭解研究對象對流感的免疫力情形，經過統計分析，得到血清保護率結果後，能幫助預測當年年底流行之流感病毒株，進一步還能評估疫情規模，再加上相關人口學資料及對照其疫苗接種紀錄，分析流感疫苗是否能提供足夠的保護力。有這些實證資料當作參考依據，加強對流行性感冒的防疫工作。<br> Abstract: Influenza virus is one of the main causes of pneumonia of the elderly and children each year. It can cause otitis media, acute bronchitis, encephalitis and other diseases. With the gene mutability of influenza virus, it often causes yearly epidemics around the world, which lead to many people being sick, and even death. Influenza vaccination is the most effective strategy to prevent influenza for now. It has been repeatedly shown that vaccination of people induce protective antibodies and reduce the severity of infection or death associated with influenza. But the actual protective effects still require thorough survey of serum epidemiologyThe aim of this project is to investigate the influenza seroepidemiology in preschool children to correlate with the extent of yearly influenza epidemics. The hemagglutinin inhibition (HAI) assay will be used to detect the serum antibody of influenza virus. In order to understand the effect of influenza vaccine, we will analyze the relevant demographic data and influenza vaccination record. The results of this project will be the references for the forecast of influenza epidemic strain, epidemic scale and the policies of vaccination in the future.We will enroll 60 healthy children aged between 2 to 6 years old in this study every year. Children will be surveyed according to questionnaire and venipunctured to collect blood between August and November every year. The HAI titers against 4 strains (H1N1, H3N2, B /Vitoria, B /Yamagata) will be determined. The results of seroprotection rate will help us to predict the epidemic influenza virus strain and evaluate the scale of epidemic. Children’s serum antibody titer will be further analyzed with the relevant demographic data and influenza vaccination record to understand whether the flu vaccines provide enough immunity to protect children. These data will serve as the evidence to evaluate the effectiveness of national influenza vaccination policy.流感病毒流感疫苗抗體免疫反應血清保護率influenza virusantibody responseseroprotection rateyearly epidemicinfluenza vaccine