2023-01-012024-05-13https://scholars.lib.ntu.edu.tw/handle/123456789/653695甘藍黃葉病(Fusarium wilt)由鐮孢菌(Fusarium oxysporum f. sp. conglutinans)所引起，為影響臺灣甘藍生產的土壤傳播性病害之一。本實驗室已建立甘藍嫁接技術，將利用日本抗病品種做為根砧進行嫁接，做為新抗病品種育成前之替代解決方案；此外，將於多種品種上初步測試已開發之甘藍黃葉病抗病基因分子標誌，觀察其是否與接種實驗有相同結果。本研究於第一年已完成(1)確認接種病原菌濃度及方法(2)評估日本抗病品種及臺灣耐熱品種對臺灣黃葉病的抗病及耐病能力；第二年完成(1)確認篩選出之抗病品種於高溫下的抗病穩定性(2)確認所選抗病品種對不同黃葉病菌株之抗病性(3)以病菌土接種方式評估嫁接苗抗病及耐病能力(4)評估甘藍黃葉病抗病基因分子標誌之準確性；本年(第三年)則預計(1)選用準確性較高之分子標誌篩選更多抗病品種(2)確認新選出之抗病品種對不同黃葉病菌株之抗病性(3)實際進行田間試驗，評估抗病品種或使用抗病根砧之甘藍嫁接株於田間之抗病能力。因此，本研究利用日本抗病品種結合甘藍嫁接技術減少因臺灣黃葉病引起的減產問題；同時評估多個分子標誌之準確性，用於快速篩選抗病品種及未來分子標誌輔助選育上。 Fusarium wilt is caused by Fusarium oxysporum f. sp. conglutinans, and is one of the soil-borne pathogens that seriously affect cabbage production in Taiwan. The cabbage grafting technique had been developed in our laboratory, so it is viable to select Japanese disease-resistant cultivars as rootstocks for grafting, and use this as an alternative solution before new disease-resistant cultivars have been developed. Furthermore, the developed molecular markers for the fusarium wilt disease resistance gene of cabbage will be preliminarily tested on a variety of cultivars to observe whether they have the same results as the inoculation experiment. In the first year, this research has completed (1) confirm the concentration and method for pathogen inoculation (2) evaluate the disease resistance and tolerance of Japanese disease-resistant cultivars and Taiwan heat-tolerant cultivars to Taiwan fusarium wilt. The second year, this research has completed (1) confirm the disease resistance stability of selected disease resistant cultivars under high temperature (2) confirm the disease resistance of selected resistant cultivars to different cabbage fusarium wilt isolates (3) evaluate the disease resistance and tolerance of grafted seedlings after infested soil inoculation (4) evaluate the accuracy of molecular markers for fusarium wilt resistance gene. This year (the third year), this research is planned to (1) Use molecular markers with higher accuracy to screen more resistant cultivars (2) confirm the disease resistance of newly selected resistant cultivars to different cabbage fusarium wilt isolates (3) conduct field trials to evaluate the disease resistance of disease-resistant cultivars and/or grafted plants with disease-resistant rootstocks. Therefore, this research will combine Japanese disease-resistant cultivars with cabbage grafting technology to reduce yield reduction problems caused by fusarium wilt in Taiwan. Meanwhile, the accuracy of multiple molecular markers was evaluated and used for rapid screening of disease-resistant cultivars and future molecular marker-assisted selection.甘藍;嫁接;甘藍黃葉病;分 子標誌;Cabbage;Grafting;Cabbage fusarium wilt;Molecular marker