2010-08-012024-05-14https://scholars.lib.ntu.edu.tw/handle/123456789/657749摘要：牙科植體使用純鈦或是鈦合金雖然可以形成穩定的骨整合，但在骨質鬆軟區域仍需有較長的癒合時間，以及較高的失敗率。早期機械性的增加表面粗糙度後可以有效的縮短骨整合的時間， 但是在骨質不緻密的區堿仍需要較長的癒合時間，而近幾年研發的重心是植體的表面處理，以加速骨整合的速度，本實驗旨在研發新一代的人工牙根表面處理，期能減少癒合所需時間，不同於過去機械性的增加表面粗糙度的方法，新一代的表面處理會有生物性的特質，可以加強骨細胞的附著或是促進骨細胞的分生來加速骨整合的時間。實驗大致分成四個階段，目前正進行第一階段已於97 年獲國科會補助已完成，藉由噬箘顯現法（Phage Display）找出數個與鈦金屬有強烈結合能力的胜肽。此次提出的三年計畫為後續的第二至四階段,第一年計畫合成鈦金屬結合胜肽與促進骨癒合的分子的融合蛋白看是否對體外的造骨細胞有影響，此外委託國內廠商提供國產人工牙根在狗的腿骨進行前臨床測試，建立出實驗動物流程及人工牙根的適用性。第二及三年則為動物實驗與生物相容性測試，依據目前計畫及新申請第一年實驗所得的結果，將進行融合蛋白生物相容性相關測試評估生物安全後，將此結合在國產植體表面，並植入狗的顎骨中，測試此融合蛋白是否會對骨整合有影響。此種表面處理的方式為國內外首創，尚未有類似的實驗發表過，研究潛力高，且實驗的結果對未來的臨床應用有直接的貢獻，具有極大的商業價值。<br> Abstract: Titanium and its alloys were mostly proposed and have been successfullyused in reconstructive surgery and prosthetic treatment. Since theintroduction of titanium root-form dental implant in 1960’s, titanium hasbecome a widely used treatment modality in rehabilitation of completely orpartially edentulous patients. However, dental implants may fail because ofsome problems or complications such as inadequate bone healing,progressive marginal bone loss, recession of marginal mucosa, or loss ofosseointegration. It is known that the early osseointegration and thebone-implant interaction play an essential role that can determine theoutcomes of dental implant therapy. To gain a better outcome in dental implantprocedure, many studies attended to develop different modification of titaniumimplant. With the increased understanding of the function of bone-healingrelated proteins, applying bone-healing factors to the biochemical modificationof titanium becomes potential therapeutics to promote the outcomes of dentalimplant surgery. The major strategy of this study is to find mediators that canbe used to connect the titanium implant surface and bone-healing promotingfactors. The incorporation of the titanium implant and bone-healing promotingfactors could efficiently progress the bone-healing process occurred in theimplant-surrounding tissue. Small peptides with higher biocompatibility couldbe appropriate mediators to connect the titanium implant surface.The objective of this study is to identify small peptides that can be fusedwith target bone-healing promoting factors and connected to the surface oftitanium implants. We use the phage display system, a powerful highthrough-put screening system for small peptides, to find appropriatetitanium-binding peptides. Both in vitro and animal experiments will beperformed to validate their binding affinity and specificity as well as to assesstheir application outcome in the dental implant surgery. It is expected that suchtitanium-binding fusion protein coming out in this study will become thepowerful tools to promote the dental implant surgery in the future.