Huang, Chao-YangChao-YangHuangLok, Ying-YungYing-YungLokLin, Chia-HuiChia-HuiLinLai, Szu-LiangSzu-LiangLaiWu, Yen-YuYen-YuWuHu, Chih-YungChih-YungHuLiao, Chu-BinChu-BinLiaoHo, Chen-HsuanChen-HsuanHoChou, Yu-PingYu-PingChouHsu, Yi-HsuanYi-HsuanHsuLo, Yu-HsunYu-HsunLoEdward CHERN2023-06-052023-06-052023-04-271664-3224https://scholars.lib.ntu.edu.tw/handle/123456789/631754Monoclonal antibodies (mAbs) and their derivatives are the fastest expanding category of pharmaceuticals. Efficient screening and generation of appropriate therapeutic human antibodies are important and urgent issues in the field of medicine. The successful in vitro biopanning method for antibody screening largely depends on the highly diverse, reliable and humanized CDR library. To rapidly obtain potent human antibodies, we designed and constructed a highly diverse synthetic human single-chain variable fragment (scFv) antibody library greater than a giga in size by phage display. Herein, the novel TIM-3-neutralizing antibodies with immunomodulatory functions derived from this library serve as an example to demonstrate the library's potential for biomedical applications.enTIM-3; antibody therapeutics; phage display; single-chain variable fragment (scFv); synthetic antibody library[SDGs]SDG3journal article10.3389/fimmu.2023.1089395371801552-s2.0-85159183928WOS:000986125500001https://api.elsevier.com/content/abstract/scopus_id/85159183928