LI-HUI TSENGPEI-JER CHENLin M.-T.Singleton K.Martin E.G.Yen A.-H.Martin P.J.Hansen J.A.2021-07-032021-07-0320020022-1759https://www.scopus.com/inward/record.uri?eid=2-s2.0-84983723321&doi=10.1016%2fS0022-1759%2802%2900165-5&partnerID=40&md5=4917696f453b8f5d74a92495a7a436d1https://scholars.lib.ntu.edu.tw/handle/123456789/568769The interleukin-1 (IL-1) gene complex consists of the IL-1α, IL-1β and IL-1 receptor antagonist genes. Single-nucleotide polymorphisms (SNP) in all three genes have been associated with human diseases. In this study, primers containing mismatches at 1-3 nucleotide positions were designed to incorporate a restriction site for endonuclease AlwNI or XcmI in the presence of allele-specific nucleotides at the polymorphic positions. Based on this technique, a simple and robust multiplex polymerase chain reaction/restriction fragment length polymorphism (multiplex PCR/RFLP) assay was developed to determine simultaneously three to four informative SNPs (IL-1β/+3954, IL-1β/-511 and IL-1Ra/9261 or IL-1α/-889, IL-1β/-31, IL-1β/5810 and IL-1Ra/11100 SNPs) in the IL-1 gene complex. ? 2002 Elsevier Science B.V. All rights reserved.[SDGs]SDG3endonuclease; interleukin 1 complex; interleukin 1 receptor blocking agent; interleukin 1alpha; interleukin 1beta; nucleotide; unclassified drug; allele; article; comparative study; genetic analysis; genotype; nucleotide sequence; polymerase chain reaction; priority journal; restriction fragment length polymorphism; restriction mapping; restriction site; single nucleotide polymorphismjournal article10.1016/S0022-1759(02)00165-52-s2.0-84983723321