Hsieh H.-YLuo J.-XShen Y.-HLo S.-CHsu Y.-CTahara HFan Y.-JWei P.-KSheen H.-J.HORN-JIUNN SHEEN2022-03-222022-03-22202209254005https://www.scopus.com/inward/record.uri?eid=2-s2.0-85121990430&doi=10.1016%2fj.snb.2021.131327&partnerID=40&md5=2e09b45763c2ebce02bfaec9b7bf6ceahttps://scholars.lib.ntu.edu.tw/handle/123456789/600267Epstein-Barr virus (EBV) positivity is one of the indexes for diagnosing nasopharyngeal carcinoma (NPC). Moreover, systemic inflammatory responses can easily be triggered in patients who are both EBV- and coronavirus disease 2019 (COVID-19)-positive. Development of rapid and highly sensitive EBV screening methods has become important. In this study, a nanofluidic preconcentrator integrated with a nanoslit Fano resonance biosensor was developed to detect latent membrane protein 1 (LMP1) for an EBV diagnosis. Through nanoimprinting and aluminum deposition, the low-cost nanoslit plasmonic sensing chip can be mass-produced. The nanoporous membrane was patterned on a sensing chip as an ion selective channel to concentrate LMP1 proteins. Anti-LMP1 immunoglobulin G was then modified to a sensing chip to immunosense LMP1. The Fano resonant spectrum of the capped nanoslit array produced a transmission peak followed by a dip. We recorded and analyzed the spectrum using four methods, including area, center of mass, peak value, and dip value methods. With preconcentration, a limit of detection (LOD) of 100 pg/ml and a sensing range of 100 pg/ml to 10 ?g/ml was achieved using the peak value. ? 2021Epstein-Barr virus (EBV)Latent membrane protein 1Localized surface plasmon resonanceNanofluidic preconcentrationAluminumAluminum coatingsCoronavirusDepositionDiagnosisNanoimprint lithographyNanostructuresProteinsEpstein-Barr virusMembrane proteinsNanoslitsPre-concentrationPreconcentratorsSensing chipNanofluidicsjournal article10.1016/j.snb.2021.1313272-s2.0-85121990430