2023-01-012024-05-13https://scholars.lib.ntu.edu.tw/handle/123456789/653367多纖毛細胞分布於體內多種器官組織，其纖毛可長時間朝同一方向運動、負責不同生理功能。而多纖毛細胞分化不全可導致多種遺傳疾病。呼吸道上皮多纖毛細胞具有排除呼吸道塵埃、異物以及黏液分泌細胞所分泌之黏液的功能，在人工氣管的建構上扮演不可或缺的角色。前腦室管膜內襯多纖毛細胞則是被觀察到有可能於腦中風後出現纖毛功能缺損、導致腦室下緣表面之腦脊液失去正常流動進而造成生理上的病態、甚至影響後續治療。本子計畫欲藉由先前已建立於活體外、經由調控細胞內生性因子及微環境中的外在因子來促成將多能幹細胞分化為多纖毛細胞的平台來探討其分化機制，並期望所得知識在未來有助於與氣管再生及腦中風神經修復的轉譯醫學應用發展。 Multi-ciliated cells distributed in different organs within our body. They play different physiological roles by continuously moving toward a fixed direction. Deficiency in multi-ciliated cell differentiation resulted in a syndrome call ciliopathy. In the respiratory epithelial, ciliated cell removes dusts and mucus to keep the tract function normally. In the lining of the ventricles, ciliated cells produce current of the spinal fluid to keep the ependymal cells correctly differentiated. Therefore, to produce and to in vitro differentiate functional ciliated cells can be valuable to the construction of artificial trachea and the development therapeutics benefit patients under stroke conditions. The current project is focused on using our pre-existing model, which allows in vitro multi-ciliated cell differentiation from pluripotent stem cells, to discuss intrinsic- and extrinsic-factors involved in this differentiation process.多能幹細胞;多纖毛細胞;體外分化;pluripotent stem cell;multi-ciliated cell;in vitro differentiation