Wan, CHCHWanBauer, B.A.B.A.BauerPintel, D.J.D.J.PintelCHO-HUA WAN2011-02-152018-07-092011-02-152018-07-092006-01http://ntur.lib.ntu.edu.tw//handle/246246/226984Two newly recognized parvovirus species, rat parvovirus 1 (RPV-1) and rat minute virus 1 (RMV-1), were recently identified in naturally infected rats. In this study, two polymerase chain reaction (PCR) assays were developed to specifically detect RPV-1 and RMV-1. The RPV-1 PCR assay amplified the expected 487-bp deoxyribonucleic acid (DNA) fragment only in the presence of RPV-1 DNA; the RMV-1 PCR assay amplified the expected 843-bp product only from RMV-1 DNA, not from other rodent parvoviruses. The RPV-1 and the RMV-1 PCR assays detected approximately 18 and 70 copies of DNA template, respectively. These two PCR assays were shown to be sensitive, specific and rapid methods for detecting RPV-1 and RMV-1 infections in rats. These assays may also be valuable for evaluation of biological specimens for parvovirus contamination. ? Laboratory Animals Ltd.en-US[SDGs]SDG3virus DNA; animal tissue; article; controlled study; diagnostic test; diagnostic value; DNA extraction; DNA sequence; DNA template; evaluation; gene amplification; nonhuman; Parvovirus; polymerase chain reaction; rat; rat minute virus 1; rat parvovirus 1; sensitivity and specificity; viral contamination; virus detection; virus gene; virus infection; virus morphology; Animals; Animals, Newborn; Biological Assay; Cricetinae; DNA Primers; DNA, Viral; Humans; Mice; Minute virus of mice; Parvoviridae Infections; Parvovirus; Polymerase Chain Reaction; Rats; Rodent Diseases; Sequence Analysis, DNA; Animalia; DNA viruses; Murinae; Parvovirus; Reindeer papillomavirus; rodent parvovirusesjournal article10.1258/0023677067754044082-s2.0-33644787304