泌尿科Gray, Margaret A.Margaret A.GrayWang, Chung-ChengChung-ChengWangSacks, Michael S.Michael S.SacksYoshimura, NaokiNaokiYoshimuraChancellor, Michael B.Michael B.ChancellorNagatomi, JiroJiroNagatomi2009-12-232018-07-112009-12-232018-07-112008http://ntur.lib.ntu.edu.tw//handle/246246/175131OBJECTIVES To investigate time-course changes in the expression of select genes and extracellular matrix proteins in the bladder of rats with streptozotocin-induced diabetes , so as to examine the mechanisms underlying changes in mechanical properties of the bladder due to diabetic cystopathy. METHODS Female Sprague-Dawley rats were injected with streptozotocin (65 mg/kg). Rats that were fed with 5% sucrose in drinking water served as diuretic controls, in addition to normal control rats. At the end of 2, 4, and 8 weeks, total ribonucleic acid (RNA) isolated from the detrusor layer of the bladders was reverse transcribed, and then complementary deoxyribonucleic acid was amplified with polymerase chain reaction primer sets for type I Collagen, type III Collagen, tropoelastin, and transforming growth factor beta 1 (TGF-beta-1). Collagen and elastin contents of the bladders were quantified with commercially available assays. RESULTS Both diabetic and diuretic rat bladders exhibited significantly (P <0.05) lower expression of type I Collagen and TGF-beta-1 messenger RNA (mRNA) compared with normal controls at all time points tested. In contrast, downregulation of type III Collagen mRNA expression in both diabetic and diuretic groups was seen at 4 and 8 weeks. Furthermore, tropoelastin mRNA expression in the diabetic rat bladders was , compared with normal and diuretic rats, significantly (P <0.05) greater at 2 weeks. Both diabetic and diuretic rat bladders exhibited significantly (P <0.05) decreased Collagen and increased elastin protein content at 2 and 8 weeks. CONCLUSIONS The results of the present study suggest that increases in compliance of the bladders in diabetic cystopathy result not only from diuresis-driven reduction of Collagen synthesis but also from increased elastin synthesis.en-US[SDGs]SDG3collagen type 1; collagen type 3; complementary DNA; RNA; streptozocin; transforming growth factor beta1; tropoelastin; animal experiment; animal model; article; controlled study; diabetes mellitus; down regulation; female; gene expression; nonhuman; priority journal; protein content; rat; reverse transcription polymerase chain reaction; Animals; Diabetes Mellitus, Experimental; Extracellular Matrix Proteins; Female; Gene Expression; Rats; Rats, Sprague-Dawley; Time Factors; Urinary Bladder10.1016/j.urology.2007.11.054