Lee C.-HLin W.-SYang M.-CHo C.-TMIN-HSIUNG PAN2021-07-262021-07-26202016052471https://www.scopus.com/inward/record.uri?eid=2-s2.0-85100405789&doi=10.6578%2fTJACFS.2020010-58%284-5%29.0004&partnerID=40&md5=92beaf538cc27e7421a7103de22c82fehttps://scholars.lib.ntu.edu.tw/handle/123456789/572884Cancer metastasis, the terminal stage of cancer development, is accounting for approximately 90% of all human cancer mortalities. Previous research has suggested that theasinensin A (TSA) has anti-inflammatory effects; however, its potential for inhibiting colon cancer metastasis remains to be unclear. Therefore, in this research, we focused on investigating the mechanism of inhibitory effects of TSA on TPA-induced colon cancer cell migration. Previous studies show that matrix metalloproteinase-9 (MMP-9) and C-C motif ligand 2 (CCL2) are critical factors that cause cancer cell intravasation and extravasation. In our research, we tried to figure out if TSA can downregulate TPA-induced protein expression and activity of MMP-9 and CCL2 mRNA expression in human colon HT-29 cells. Our results showed that TSA effectively inhibited TPA-induced epithelial-mesenchymal transition (EMT), mesenchymal-epithelial transition (MET), cell anchorage-independent growth, and cell migration in HT-29 cells. Moreover, TSA may downregulate TPA-induced expression of MMP-9 and CCL2 via inhibiting TPA-induced phosphorylation of ERK.1/2 and p38 and AP-1 activation whereby further inhibit cancer cell migration. Our findings suggested that TSA could be a potential compound for preventing colon cancer metastasis. ? 2020 Chinese Agricultural Chemical Society. All rights reserved.C-C motif ligand 2 (CCL2); Cancer metastasis; Matrix metalloproteinase-9 (MMP-9); Theasinensin A (TSA)[SDGs]SDG3journal article10.6578/TJACFS.2020010-58(4-5).00042-s2.0-85100405789