Inhibitory effect of CDA-II, a urinary preparation, on aflatoxin B 1 -induced oxidative stress and DNA damage in primary cultured rat hepatocytes

Lin W.C.Liao Y.C.Liau M.C.Lii C.K.Sheen L.Y.2019-07-112019-07-1120062786915https://scholars.lib.ntu.edu.tw/handle/123456789/413588The effects of CDA-II (cell differentiation agent II; a urinary preparation) on both aflatoxin B 1 (AFB 1 )-induced cell injury and DNA damage were investigated using cultured rat hepatocytes. CDA-II was able to suppress both the lipid peroxidation and lactate dehydrogenase leakage induced by AFB 1 . Glutathione (GSH) depletion by AFB 1 was replenished by CDA-II treatment. Under these experimental conditions, CDA-II enhanced the activity of GSH peroxidase, but not GSH S-transferase. By evaluation of unscheduled DNA synthesis, CDA-II reduced AFB 1 -induced DNA damage in hepatocyte cultures. These findings suggest that CDA-II can inhibit cytotoxicity of AFB 1 through enhancing the activity of GSH peroxidase and preventing GSH depletion. ? 2005 Elsevier Ltd. All rights reserved.A urinary preparationAflatoxin B 1CDA-IIDNA damageLipid peroxidationPrimary rat hepatocytesInhibitory effect of CDA-II, a urinary preparation, on aflatoxin B 1 -induced oxidative stress and DNA damage in primary cultured rat hepatocytesjournal article10.1016/j.fct.2005.08.0292-s2.0-33644867938https://www.scopus.com/inward/record.uri?eid=2-s2.0-33644867938&doi=10.1016%2fj.fct.2005.08.029&partnerID=40&md5=ed832daedf35e2faa2ae5465bc5f95ea