Chang, Cherng ShyangCherng ShyangChangLiao, Yi ChuYi ChuLiaoHuang, Chih TingChih TingHuangLin, Chiao MeiChiao MeiLinCheung, Chantal Hoi YinChantal Hoi YinCheungRuan, Jhen WeiJhen WeiRuanYu, Wen HsuanWen HsuanYuTsai, Yi TingYi TingTsaiLin, I. JungI. JungLinHuang, Chien HsunChien HsunHuangLiou, Jong ShianJong ShianLiouChou, Ya HsienYa HsienChouChien, Hung JenHung JenChienChuang, Hsiao LiHsiao LiChuangHSUEH-FEN JUANHuang, Hsuan ChengHsuan ChengHuangChan, Hong LinHong LinChanLiao, Yu ChiehYu ChiehLiaoTang, Shiue ChengShiue ChengTangSu, Yu WenYu WenSuTan, Tse HuaTse HuaTanBäumler, Andreas J.Andreas J.BäumlerKao, Cheng YuanCheng YuanKao2021-12-142021-12-142021-11-2322111247https://scholars.lib.ntu.edu.tw/handle/123456789/589890Strengthening the gut epithelial barrier is a potential strategy for management of gut microbiota-associated illnesses. Here, we demonstrate that dual-specificity phosphatase 6 (Dusp6) knockout enhances baseline colon barrier integrity and ameliorates dextran sulfate sodium (DSS)-induced colonic injury. DUSP6 mutation in Caco-2 cells enhances the epithelial feature and increases mitochondrial oxygen consumption, accompanied by altered glucose metabolism and decreased glycolysis. We find that Dusp6-knockout mice are more resistant to DSS-induced dysbiosis, and the cohousing and fecal microbiota transplantation experiments show that the gut/fecal microbiota derived from Dusp6-knockout mice also confers protection against colitis. Further culturomics and mono-colonialization experiments show that one gut microbiota member in the genus Duncaniella confers host protection from DSS-induced injury. We identify Dusp6 deficiency as beneficial for shaping the gut microbiota eubiosis necessary to protect against gut barrier-related diseases.barrier integrity | DUSP6 | experimental colitis | gut microbiota | leaky gutbarrier integrity; DUSP6; experimental colitis; gut microbiota; leaky gut[SDGs]SDG3dual specificity phosphatase 6; transcriptome; aged; animal cell; Article; bacterium identification; Caco-2 cell line; clinical article; colitis; colon epithelium; controlled study; dextran sulfate sodium-induced colitis; Dusp6 gene; dysbiosis; fecal microbiota transplantation; gene identification; gene knockout; gene mutation; glucose metabolism; glycolysis; histology; human; human cell; intestine flora; male; microvillus; mitochondrion; mouse; nonhuman; oxygen consumption; protein phosphorylation; real time polymerase chain reaction; RNA sequencing; tight junction; Western blottingjournal article10.1016/j.celrep.2021.1100162-s2.0-85119438955https://api.elsevier.com/content/abstract/scopus_id/85119438955