分子醫學研究所Loyola, A.A.LoyolaHuang, J.-Y.J.-Y.HuangLeRoy, G.G.LeRoyHu, S.S.HuWang, Y.-H.Y.-H.WangDonnelly, R. J.R. J.DonnellyLane, W. S.W. S.LaneLee, S.-C.S.-C.LeeReinberg, D.D.Reinberg呂勝春2008-12-222018-07-092008-12-222018-07-092003http://ntur.lib.ntu.edu.tw//handle/246246/92451The human ISWI-containing factor RSF (for remodeling and spacing factor) is composed of two subunits: the ATPase hSNF 2H and p325 (Rsf-1), a protein encoded by a novel human gene. We previously showed that RSF mediates nucleosome deposition and generates regularly spaced nucleosome arrays. Here we report the characterization of the largest subunit of RSF, Rsf-1. We found that Rsf-1 is a highly acidic protein containing a plant homology domain. The present study includes the cloning of Rsf-1, the preparation of recombinant RSF, and the dissection of the role of each subunit in the chromatin assembly reaction. The sequence of the gene for Rsf-1 includes a recently characterized cDNA, HBXAP; postulated to be involved in the transcriptional regulation of the hepatitis B virus. HBXAP actually contains a 252-amino-acid truncation of the amino terminus of Rsf-1. Finally, comparison of HBXAP and Rsf-1 properties shows that they are functionally different.en-US[SDGs]SDG3