SZU-MIN HSIEHSHAN-CHWEN CHANG2020-12-292020-12-2920060022-1767https://www.scopus.com/inward/record.uri?eid=2-s2.0-33645767911&doi=10.4049%2fjimmunol.176.8.4530&partnerID=40&md5=bd82664af0dcb3e51b43717d831a6857https://scholars.lib.ntu.edu.tw/handle/123456789/535504Hypercytokinemia has been considered to be responsible for hemophagocytosis and multiorgan failure in severe human cases of H5N1 viral infection. In this study, we show a recombinant hemagglutinin (H5) from a H5N1 virus may suppress the perform expression and reduce cytotoxicity of human CD8+ T cells to kill H5-bearing cells. The persistence of H5-presenting cells provides sustained stimulation and leads to a marked lymphoproliferation and IFN-γ hyperproduction. We propose the IFN-γ hyperproduction may explain macrophage overactivation and sub-sequent hypercytokinemia and hemophagocytosis in severe human cases of avion influenza. Copyright ? 2006 by The American Association of Immunologists, Inc.[SDGs]SDG3gamma interferon; hemagglutinin; perforin; gamma interferon; hemagglutinin, avian influenza A virus; influenza vaccine; Influenza virus hemagglutinin; membrane protein; perforin; pore forming cytotoxic protein; recombinant protein; adult; article; avian influenza; avian influenza virus; CD8+ T lymphocyte; controlled study; cytotoxicity; human; human cell; human experiment; Influenza virus; interferon production; lymphocyte proliferation; macrophage; multiple organ failure; normal human; phagocytosis; priority journal; protein expression; virus infection; virus strain; biosynthesis; cell proliferation; cytology; dendritic cell; immunology; in vitro study; Influenza virus A H5N1; kinetics; lymphocyte activation; Adult; CD8-Positive T-Lymphocytes; Cell Proliferation; Cytotoxicity, Immunologic; Dendritic Cells; Hemagglutinin Glycoproteins, Influenza Virus; Humans; Influenza A Virus, H5N1 Subtype; Influenza Vaccines; Interferon Type II; Kinetics; Lymphocyte Activation; Membrane Glycoproteins; Pore Forming Cytotoxic Proteins; Recombinant ProteinsCutting edge: Insufficient perforin expression in CD8+ T cells in response to hemagglutinin from avian influenza (H5N1) virusjournal article10.4049/jimmunol.176.8.453016585542