SZU-HUA PANKANG-YI SUSpiessens B.Kusuma N.Delahaye N.F.Gruselle O.Myo A.de Creus A.Louahed J.Chang G.-C.SUNG-LIANG YUPAN-CHYR YANG2021-06-042021-06-0420171743-7555https://www.scopus.com/inward/record.uri?eid=2-s2.0-84992456076&doi=10.1111%2fajco.12586&partnerID=40&md5=97773c15bb3436b92767a1b6ba02f0cbhttps://scholars.lib.ntu.edu.tw/handle/123456789/565095Aim: To determine the frequency of expression of the tumor-associated antigens (TAAs) melanoma-associated antigen A3 (MAGE-A3) and preferentially expressed antigen of melanoma (PRAME) and the rate of EGFR mutations in a Taiwanese non–small cell lung cancer (NSCLC) population including only adenocarcinomas and squamous cell carcinomas. Furthermore, to investigate associations between TAA expression and EGFR mutations and to evaluate these TAAs as prognostic markers for overall survival. The occurrence of single nucleotide polymorphisms in MAGEA3 and PRAME was also assessed. Methods: Archival fresh-frozen tumor tissue specimens were tested by quantitative reverse transcription polymerase chain reaction assays to detect MAGE-A3 and PRAME expression. EGFR mutations were detected by mass spectroscopy and single nucleotide polymorphisms by gene sequencing. Results: Of the 156 adenocarcinomas examined, 3.3% expressed MAGE-A3, 32.2% expressed PRAME and 62.8% had EGFR mutations. Of the 128 squamous cell carcinomas, 29.8% expressed MAGE-A3, 59.2% expressed PRAME and 20.5% harbored EGFR mutations. TAA expression was similar across subgroups determined by patient or tumor characteristics. There was no association between TAA expression and EGFR mutation status and TAA expression was found not to be a prognostic marker for survival. Single nucleotide polymorphisms were identified, one of which with a possible impact on MAGE-A3 expression. Conclusions: In this NSCLC population, expression of MAGE-A3 and PRAME was more frequent in squamous cell carcinomas than in adenocarcinomas tumors. EGFR mutations were not associated with TAA expression for either histology and were three times more frequent in adenocarcinomas than in squamous cell carcinomas tumors. ? 2016 The Authors. Asia-Pacific Journal of Clinical Oncology Published by John Wiley & Sons Australia, Ltd.[SDGs]SDG3complementary DNA; epidermal growth factor receptor; melanoma antigen 3; preferentially expressed antigen of melanoma; RNA; tumor antigen; unclassified drug; MAGEA3 protein, human; PRAME protein, human; tumor antigen; tumor marker; tumor protein; adult; aged; Article; cancer tissue; EGFR gene; female; gene expression; gene mutation; gene sequence; human; human tissue; MAGE A3 gene; major clinical study; male; mass spectrometry; non small cell lung cancer; overall survival; PRAME gene; priority journal; retrospective study; reverse transcription polymerase chain reaction; RNA extraction; single nucleotide polymorphism; Taiwan; Taiwanese; genetic polymorphism; genetics; lung tumor; metabolism; middle aged; mutation; non small cell lung cancer; pathology; Adult; Aged; Antigens, Neoplasm; Biomarkers, Tumor; Carcinoma, Non-Small-Cell Lung; Female; Humans; Lung Neoplasms; Male; Middle Aged; Mutation; Neoplasm Proteins; Polymorphism, Genetic; Retrospective Studies; Taiwanjournal article10.1111/ajco.12586275192862-s2.0-84992456076