Chang T-T.MING-KWANG SHYUMIN-CHUAN HUANGHsu C-C.Yeh S-Y.MEI-RU CHENCHUN-JUNG LIN2020-03-022020-03-0220111543-8384https://www.scopus.com/inward/record.uri?eid=2-s2.0-79952979127&doi=10.1021%2fmp100137q&partnerID=40&md5=6ecbcc4db9b37af10ba582231e6fff85https://scholars.lib.ntu.edu.tw/handle/123456789/467193The objective of the present study was to investigate the effects of hypoxia on placental expression of OCTN2 and PPARR. OCTN2 and PPARRα expression in the human placenta in the presence or absence of preeclampsia was examined by immunohistochemical (IHC) analysis, Western blotting, and quantitative polymerase chain reaction (qPCR). Effects of hypoxia on the expression of OCTN2 and PPARRα in human placental explants and human choriocarcinoma BeWo cells were examined by Western blotting and qPCR analyses. IHC, Western blot, and qPCR studies showed that OCTN2 and PPARR protein and mRNA levels were lower in syncytiotrophoblasts from preeclamptic human placentas than in those from normal placentas. Hypoxic treatment caused a decrease in OCTN2 and PPARRα expression in human placental explants and in BeWo cells. WY14643, a PPARRα agonist, caused an increase in OCTN2 expression in BeWo cells under hypoxic conditions. In conclusion, under hypoxic conditions, placental OCTN2 is down-regulated through PPARα-mediated pathways. ? 2011 American Chemical Society.[SDGs]SDG3amino acid receptor; carnitine; forskolin; hypoxia inducible factor 1alpha; hypoxia inducible factor 2alpha; messenger RNA; OCTN2 protein; peroxisome proliferator activated receptor alpha; pirinixic acid; protein; retinoid X receptor alpha; syncytin; unclassified drug; article; cancer cell culture; carcinoma cell; choriocarcinoma; controlled study; down regulation; explant; female; gene expression; human; human cell; human tissue; hypoxia; immunohistochemistry; in vitro study; male; placenta; polymerase chain reaction; preeclampsia; priority journal; protein expression; quantitative analysis; syncytiotrophoblast; Western blotting; Adult; Basic Helix-Loop-Helix Transcription Factors; Blotting, Western; Carnitine; Cell Hypoxia; Cells, Cultured; Female; Forskolin; Gene Expression; Humans; Hypoxia-Inducible Factor 1, alpha Subunit; Immunohistochemistry; Male; Organic Cation Transport Proteins; Placenta; Polymerase Chain Reaction; PPAR alpha; Pregnancy; Tissue Culture Techniquesjournal article10.1021/mp100137q211259922-s2.0-79952979127